Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BSG32_RS16245 Genome accession   NZ_CP022552
Coordinates   3219887..3220351 (+) Length   154 a.a.
NCBI ID   WP_021450016.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain MAVP-R     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3214887..3225351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSG32_RS16230 (BSG32_16240) pdhR 3216919..3217686 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  BSG32_RS16235 (BSG32_16245) ampD 3218092..3218643 (-) 552 WP_005462574.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BSG32_RS16240 (BSG32_16250) nadC 3218736..3219623 (+) 888 WP_021450015.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BSG32_RS16245 (BSG32_16255) pilA 3219887..3220351 (+) 465 WP_021450016.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  BSG32_RS16250 (BSG32_16260) pilB 3220351..3222036 (+) 1686 WP_021450017.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BSG32_RS16255 (BSG32_16265) pilC 3222060..3223283 (+) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  BSG32_RS16260 (BSG32_16270) pilD 3223348..3224217 (+) 870 WP_021450018.1 A24 family peptidase Machinery gene
  BSG32_RS16265 (BSG32_16275) coaE 3224218..3224832 (+) 615 WP_021450019.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15734.80 Da        Isoelectric Point: 4.1438

>NTDB_id=201751 BSG32_RS16245 WP_021450016.1 3219887..3220351(+) (pilA) [Vibrio parahaemolyticus strain MAVP-R]
MKHSKQKKQQGFTLIELMIVVAIIGVLSAFAIPAYSDYTQRTRVAGAAAGISGFKTAVAMCAQDLGQLTGCDQATNDIPA
TIATGNDGATISYVDAVTVADGVITMVTTGVDDAGTLLELEFTPTIGNGVVQWDIAGTGCSDTTPGRGIDCSGN

Nucleotide


Download         Length: 465 bp        

>NTDB_id=201751 BSG32_RS16245 WP_021450016.1 3219887..3220351(+) (pilA) [Vibrio parahaemolyticus strain MAVP-R]
ATGAAACACAGTAAACAGAAAAAACAACAAGGTTTTACCTTGATTGAATTGATGATTGTCGTGGCGATTATCGGGGTATT
ATCTGCTTTTGCTATCCCAGCATACTCAGACTACACACAACGTACGCGAGTAGCCGGAGCCGCGGCTGGTATTAGCGGTT
TCAAAACAGCAGTTGCAATGTGCGCACAGGATCTTGGCCAGCTTACAGGCTGCGACCAAGCTACTAATGATATTCCAGCA
ACAATCGCTACTGGTAATGACGGCGCGACTATTTCATATGTAGATGCGGTTACCGTCGCTGACGGCGTTATTACAATGGT
AACTACAGGTGTTGATGATGCAGGTACTTTACTAGAACTTGAGTTTACTCCAACTATTGGCAACGGTGTCGTTCAGTGGG
ATATTGCCGGTACAGGATGTAGCGACACTACTCCTGGCCGCGGCATTGACTGTTCAGGTAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

43.284

87.013

0.377
  pilA2 Legionella pneumophila strain ERS1305867

43.609

86.364

0.377
  pilA Vibrio parahaemolyticus RIMD 2210633

47.899

77.273

0.37
  comP Acinetobacter baylyi ADP1

36.601

99.351

0.364

Multiple sequence alignment