Detailed information NTG0200668

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BJK08_RS05075
• Genome accession: NZ_CP017602
• Coordinates: 1104074..1105138 (+)
• Length: 354 a.a.
• NCBI ID: WP_011213353.1
• Uniprot ID: A0A3A6VL78
• Organism: Legionella pneumophila strain D5945
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1099074..1110138

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BJK08_RS05065 (BJK08_04960)	-	1100116..1102500 (-)	2385	WP_011213351.1	penicillin-binding protein 1A	-
BJK08_RS05070 (BJK08_04965)	-	1102650..1103657 (-)	1008	WP_011213352.1	hypothetical protein	-
BJK08_RS05075 (BJK08_04970)	pilM	1104074..1105138 (+)	1065	WP_011213353.1	type IV pilus assembly protein PilM	Machinery gene
BJK08_RS05080 (BJK08_04975)	pilN	1105142..1105690 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
BJK08_RS05085 (BJK08_04980)	pilO	1105704..1106303 (+)	600	WP_011213355.1	type 4a pilus biogenesis protein PilO	Machinery gene
BJK08_RS05090 (BJK08_04985)	pilP	1106300..1106884 (+)	585	WP_011213356.1	pilus assembly protein PilP	Machinery gene
BJK08_RS05095 (BJK08_04990)	pilQ	1106888..1108984 (+)	2097	WP_011213357.1	type IV pilus secretin PilQ	Machinery gene
BJK08_RS05100 (BJK08_04995)	aroK	1109430..1109957 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38788.79 Da
• Isoelectric Point: 5.0367

>NTDB_id=200668 BJK08_RS05075 WP_011213353.1 1104074..1105138(+) (pilM) [Legionella pneumophila strain D5945]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKRLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=200668 BJK08_RS05075 WP_011213353.1 1104074..1105138(+) (pilM) [Legionella pneumophila strain D5945]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAGATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCACCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: A0A3A6VL78

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.718	100	0.997
comM	Acinetobacter nosocomialis M2	42.655	100	0.427
pilM	Acinetobacter baumannii D1279779	42.373	100	0.424
comM	Acinetobacter baylyi ADP1	40.96	100	0.41