Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BG603_RS00145 Genome accession   NZ_CP017539
Coordinates   17767..18963 (+) Length   398 a.a.
NCBI ID   WP_006248403.1    Uniprot ID   A0A249A0I8
Organism   Mannheimia haemolytica strain 1454     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1626..19622 17767..18963 within 0


Gene organization within MGE regions


Location: 1626..19622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG603_RS00025 (BG603_00025) - 1626..2051 (-) 426 WP_006248641.1 gp16 family protein -
  BG603_RS00030 (BG603_00030) - 2035..2589 (-) 555 WP_006248640.1 hypothetical protein -
  BG603_RS14220 - 2589..2759 (-) 171 WP_006248639.1 hypothetical protein -
  BG603_RS00035 (BG603_00035) - 2830..3405 (-) 576 WP_006248638.1 DUF5420 family protein -
  BG603_RS00040 (BG603_00040) - 3428..3610 (-) 183 WP_006248637.1 hypothetical protein -
  BG603_RS00045 - 3677..3823 (-) 147 WP_006248636.1 ANR family transcriptional regulator -
  BG603_RS00050 (BG603_00045) - 3934..4140 (-) 207 WP_006248635.1 hypothetical protein -
  BG603_RS00055 (BG603_00050) - 4142..4759 (-) 618 WP_006248634.1 DUF3164 family protein -
  BG603_RS00060 (BG603_00055) - 4772..4963 (-) 192 WP_006248633.1 hypothetical protein -
  BG603_RS00065 (BG603_00060) - 4985..5287 (-) 303 WP_418763728.1 hypothetical protein -
  BG603_RS00070 (BG603_00065) - 5298..6179 (-) 882 WP_006248631.1 AAA family ATPase -
  BG603_RS00075 (BG603_00070) - 6183..8120 (-) 1938 WP_006248630.1 Mu transposase C-terminal domain-containing protein -
  BG603_RS00080 (BG603_00075) - 8135..8362 (-) 228 WP_006248629.1 helix-turn-helix domain-containing protein -
  BG603_RS00085 (BG603_00080) - 8572..9300 (+) 729 WP_006248628.1 helix-turn-helix domain-containing protein -
  BG603_RS00090 (BG603_00085) - 9452..10216 (+) 765 WP_236964048.1 TDT family transporter -
  BG603_RS00095 (BG603_00090) - 10317..11567 (-) 1251 WP_006248393.1 multifunctional CCA addition/repair protein -
  BG603_RS00100 (BG603_00095) nth 11764..12396 (-) 633 WP_006248394.1 endonuclease III -
  BG603_RS00105 (BG603_00100) - 12399..13163 (-) 765 WP_006248395.1 TSUP family transporter -
  BG603_RS00110 (BG603_00105) proB 13163..14247 (-) 1085 Protein_23 glutamate 5-kinase -
  BG603_RS00120 (BG603_00115) folA 14359..14853 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  BG603_RS00125 (BG603_00120) - 14973..15203 (+) 231 WP_006248398.1 YgjV family protein -
  BG603_RS00130 (BG603_00125) radA 15258..16637 (-) 1380 WP_006248399.1 DNA repair protein RadA -
  BG603_RS00135 (BG603_00130) pilA 16818..17297 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  BG603_RS00140 (BG603_00135) - 17318..17761 (+) 444 Protein_28 pilus assembly protein PilB -
  BG603_RS00145 (BG603_00140) pilC 17767..18963 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  BG603_RS00150 (BG603_00145) - 18966..19622 (+) 657 WP_006248404.1 prepilin peptidase -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 45186.75 Da        Isoelectric Point: 9.4698

>NTDB_id=199992 BG603_RS00145 WP_006248403.1 17767..18963(+) (pilC) [Mannheimia haemolytica strain 1454]
MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIRISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQA
LSMLLQNCRNIRLYLWLNELIQHIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGTVLLGTLLLAVIFYLFFAKKSRL
VAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLNAHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGIN
PNVFGAEMAQMLEVGEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFDMGALVE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=199992 BG603_RS00145 WP_006248403.1 17767..18963(+) (pilC) [Mannheimia haemolytica strain 1454]
ATGACGAAAATCTATGAATTTCATTGGCAAGCCCAGAGCCGATTTGGGCAAAAACAGCGGGGCAAGCGGTTGGCAACGAA
TCGAGACATGTTGGAAAACCTTTTGCTGGCACAAGGTTATCAGCATATTCGGATTAGCCGAAATTTTGTGCTTTCAGCAA
ACCCGAAAAAAGAAGAGGTGAATAGCTTGCTATCACAGCTTGCATTGTTGGTTAATTCTGCCATGCCGTTAAAACAGGCA
TTATCTATGTTGTTGCAAAACTGCCGAAATATCCGCCTTTATCTATGGCTTAATGAGCTGATTCAGCATATTGAAAGTGG
CTATGCTTTTTCACAATCGTTGGAGAAATTGGGTGAATTTGTGACCAATCAGGAAATTCAGCTAATCAAAATGGGCGAAC
AAAGCGGAAAACTGGGCATTATGCTGACTAATTTAGCTGAAAGCCGAGCGAAATTGGAAAAATTGGCGAAAAAAGTGAAG
AAAATCCTGTTTTACCCGGTGATTGTGTTGCTGATTTCGCTCGCTTTATCTATCGGGCTATTGGTTTTGATTGTACCGCA
GTTTGCCCAACTTTATGGCTCAAAAGACAAAACTTTACCCCTCATTACCGATATTTTATTTACCCTTTCCGAATGCTTGA
TTGAGCAAGGCACAGTATTACTTGGCACGTTACTACTAGCGGTCATTTTTTACCTGTTTTTTGCAAAAAAGAGCAGGTTA
GTCGCAAAGTGGAAAATGGCTCTATGGGCTAAAATGCCACTGTTTAGCCGAATTATTGAGCAATCTCGCATTATCTTTTT
CAGCCAAAATATTGCCTTAATGCTGAATGCTCATATTCCGCTTGATGTTGCCTTGAGGTCGTTTTTATCCGAGAAAAATG
ATGACCCGATTTTACAGCAAGAAATTGGCACTATGCTTAATTTGTTACAACAAGGCTACAAATTTTCACAAGGGATCAAC
CCGAATGTGTTTGGTGCTGAGATGGCACAAATGCTTGAGGTTGGGGAGAAAAGTGGCAATGTGGCGAAAATGTGTGAGCA
TATTAGTGAGATGTACCAACAAAAATTAGATTACCAAATTGATATTTTGTCTCAGCTATTAGAACCGATGCTAATGTTGA
TAATGGGCGTGATTGTCGGCACTATTATTGTTGGGCTGTATTTACCGATTTTTGATATGGGGGCTTTGGTGGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0I8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

54

100

0.543
  pilC Haemophilus influenzae Rd KW20

42.145

100

0.425
  pilC Haemophilus influenzae 86-028NP

41.147

100

0.415

Multiple sequence alignment