Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BG603_RS00135 Genome accession   NZ_CP017539
Coordinates   16818..17297 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 1454     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1626..19622 16818..17297 within 0


Gene organization within MGE regions


Location: 1626..19622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG603_RS00025 (BG603_00025) - 1626..2051 (-) 426 WP_006248641.1 gp16 family protein -
  BG603_RS00030 (BG603_00030) - 2035..2589 (-) 555 WP_006248640.1 hypothetical protein -
  BG603_RS14220 - 2589..2759 (-) 171 WP_006248639.1 hypothetical protein -
  BG603_RS00035 (BG603_00035) - 2830..3405 (-) 576 WP_006248638.1 DUF5420 family protein -
  BG603_RS00040 (BG603_00040) - 3428..3610 (-) 183 WP_006248637.1 hypothetical protein -
  BG603_RS00045 - 3677..3823 (-) 147 WP_006248636.1 ANR family transcriptional regulator -
  BG603_RS00050 (BG603_00045) - 3934..4140 (-) 207 WP_006248635.1 hypothetical protein -
  BG603_RS00055 (BG603_00050) - 4142..4759 (-) 618 WP_006248634.1 DUF3164 family protein -
  BG603_RS00060 (BG603_00055) - 4772..4963 (-) 192 WP_006248633.1 hypothetical protein -
  BG603_RS00065 (BG603_00060) - 4985..5287 (-) 303 WP_418763728.1 hypothetical protein -
  BG603_RS00070 (BG603_00065) - 5298..6179 (-) 882 WP_006248631.1 AAA family ATPase -
  BG603_RS00075 (BG603_00070) - 6183..8120 (-) 1938 WP_006248630.1 Mu transposase C-terminal domain-containing protein -
  BG603_RS00080 (BG603_00075) - 8135..8362 (-) 228 WP_006248629.1 helix-turn-helix domain-containing protein -
  BG603_RS00085 (BG603_00080) - 8572..9300 (+) 729 WP_006248628.1 helix-turn-helix domain-containing protein -
  BG603_RS00090 (BG603_00085) - 9452..10216 (+) 765 WP_236964048.1 TDT family transporter -
  BG603_RS00095 (BG603_00090) - 10317..11567 (-) 1251 WP_006248393.1 multifunctional CCA addition/repair protein -
  BG603_RS00100 (BG603_00095) nth 11764..12396 (-) 633 WP_006248394.1 endonuclease III -
  BG603_RS00105 (BG603_00100) - 12399..13163 (-) 765 WP_006248395.1 TSUP family transporter -
  BG603_RS00110 (BG603_00105) proB 13163..14247 (-) 1085 Protein_23 glutamate 5-kinase -
  BG603_RS00120 (BG603_00115) folA 14359..14853 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  BG603_RS00125 (BG603_00120) - 14973..15203 (+) 231 WP_006248398.1 YgjV family protein -
  BG603_RS00130 (BG603_00125) radA 15258..16637 (-) 1380 WP_006248399.1 DNA repair protein RadA -
  BG603_RS00135 (BG603_00130) pilA 16818..17297 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  BG603_RS00140 (BG603_00135) - 17318..17761 (+) 444 Protein_28 pilus assembly protein PilB -
  BG603_RS00145 (BG603_00140) pilC 17767..18963 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  BG603_RS00150 (BG603_00145) - 18966..19622 (+) 657 WP_006248404.1 prepilin peptidase -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=199991 BG603_RS00135 WP_006248401.1 16818..17297(+) (pilA) [Mannheimia haemolytica strain 1454]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=199991 BG603_RS00135 WP_006248401.1 16818..17297(+) (pilA) [Mannheimia haemolytica strain 1454]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491

Multiple sequence alignment