Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BG566_RS04630 Genome accession   NZ_CP017507
Coordinates   921049..921528 (+) Length   159 a.a.
NCBI ID   WP_020831302.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 28253     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 916049..926528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG566_RS04600 (BG566_04525) - 916622..917386 (-) 765 WP_006248395.1 TSUP family transporter -
  BG566_RS04605 (BG566_04530) proB 917386..918477 (-) 1092 WP_006251346.1 glutamate 5-kinase -
  BG566_RS04610 (BG566_04535) folA 918589..919083 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  BG566_RS04615 (BG566_04540) - 919203..919433 (+) 231 WP_006251345.1 YgjV family protein -
  BG566_RS04620 (BG566_04545) radA 919488..920867 (-) 1380 WP_006251344.1 DNA repair protein RadA -
  BG566_RS04630 (BG566_04550) pilA 921049..921528 (+) 480 WP_020831302.1 prepilin peptidase-dependent pilin Machinery gene
  BG566_RS04635 (BG566_04555) pilB 921549..922952 (+) 1404 WP_006251341.1 GspE/PulE family protein Machinery gene
  BG566_RS04640 (BG566_04560) pilC 922936..924132 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  BG566_RS04645 (BG566_04565) - 924135..924791 (+) 657 WP_006248404.1 prepilin peptidase -
  BG566_RS04650 (BG566_04570) coaE 924827..925462 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  BG566_RS04655 (BG566_04575) yacG 925444..925629 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16855.17 Da        Isoelectric Point: 8.7964

>NTDB_id=199268 BG566_RS04630 WP_020831302.1 921049..921528(+) (pilA) [Mannheimia haemolytica strain 28253]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVELCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=199268 BG566_RS04630 WP_020831302.1 921049..921528(+) (pilA) [Mannheimia haemolytica strain 28253]
ATGCAAAAAATTGTACATTTTCGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTAATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACCATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTGCAAGCCTCTGCTTCGTATAAATCAGACGTTGAACTCTGTATTTACAACAGTGGCGGGCTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

57.143

88.05

0.503
  pilA Haemophilus influenzae Rd KW20

55.634

89.308

0.497

Multiple sequence alignment