Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BIZ42_RS05750 Genome accession   NZ_CP017483
Coordinates   1309509..1310567 (-) Length   352 a.a.
NCBI ID   WP_019183880.1    Uniprot ID   A0A498CGW1
Organism   Stenotrophomonas sp. LM091     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1304509..1315567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BIZ42_RS05730 (BIZ42_05730) - 1305466..1307454 (-) 1989 WP_070425824.1 type IV pilus secretin PilQ family protein -
  BIZ42_RS05735 (BIZ42_05735) - 1307475..1308011 (-) 537 WP_156785693.1 pilus assembly protein PilP -
  BIZ42_RS05740 (BIZ42_05740) - 1308008..1308682 (-) 675 WP_019183882.1 type 4a pilus biogenesis protein PilO -
  BIZ42_RS05745 (BIZ42_05745) - 1308679..1309509 (-) 831 WP_070425825.1 PilN domain-containing protein -
  BIZ42_RS05750 (BIZ42_05750) pilM 1309509..1310567 (-) 1059 WP_019183880.1 pilus assembly protein PilM Machinery gene
  BIZ42_RS05755 (BIZ42_05755) - 1310791..1313220 (+) 2430 WP_070425826.1 penicillin-binding protein 1A -
  BIZ42_RS05760 (BIZ42_05760) - 1313410..1314057 (+) 648 WP_019183878.1 hypothetical protein -
  BIZ42_RS05765 (BIZ42_05765) - 1314081..1314845 (-) 765 WP_070425827.1 alpha/beta hydrolase -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37704.24 Da        Isoelectric Point: 4.4418

>NTDB_id=198710 BIZ42_RS05750 WP_019183880.1 1309509..1310567(-) (pilM) [Stenotrophomonas sp. LM091]
MGLIPKSQSPLIGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAVNRSGTKARHAAAA
VAGSAVITKLIPMPADLDENDMEAQVELEAVNYIPYPIEEVNLDFEVLGAIPNNPEMVQVLLAASRSENVELRQSALELG
GLVARVMDVEAFAVENAFALVASELPVASDGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEVMRRYGLTYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAALTGLPDMVEEQLGVATVVANPLA
QMTLGPKVQAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=198710 BIZ42_RS05750 WP_019183880.1 1309509..1310567(-) (pilM) [Stenotrophomonas sp. LM091]
GTGGGGCTTATCCCAAAAAGCCAGTCGCCTCTTATCGGCGTCGATATCAGCTCGACTGCAGTAAAGCTGTTGCAGTTATC
CCGCAGCGGCAATCGTTTCCGCGTGGAACATTACGCCGTGGAACCACTCCCGCCGAATGCGGTCGTGGAAAAGAACATCG
TGGAGGTCGAAGCGGTCGGGGAGGCCATTCGACGTGCCGTGAACCGCTCCGGAACCAAGGCACGCCATGCGGCGGCAGCC
GTGGCCGGCTCGGCCGTGATCACCAAGCTGATCCCGATGCCCGCCGACCTGGACGAGAACGACATGGAAGCCCAGGTCGA
GCTCGAGGCGGTCAACTACATCCCGTACCCGATCGAGGAAGTGAACCTCGACTTCGAAGTGCTGGGTGCGATTCCGAACA
ACCCGGAAATGGTCCAGGTGCTGCTGGCTGCCTCGCGTTCAGAGAACGTCGAGCTGCGCCAGTCGGCACTGGAGCTGGGC
GGGCTGGTGGCGCGGGTCATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTTTGCCCTGGTCGCCAGCGAGCTGCCGGT
GGCCAGTGATGGCGTGGTCGCGCTGGTGGACATCGGCGCCACCATGACCACCCTGAATGTCCTGCGTGGCGGCCGCAGCC
TGTACAGCCGCGAACAGGTGTTCGGCGGCAAGCAGCTGACCGACGAAGTGATGCGCCGTTACGGGCTGACCTATGAAGAG
GCCGGCCTGGCCAAGCGCCAGGGCGGGCTGCCGGAAAGCTACGAGATGGAAGTGCTCGAGCCGTTCAAGGAAGCCACGGT
CCAGCAGATCAGTCGCCTGCTGCAGTTCTTCTATGCAGGCAGCGAGTTCAACCGGGTCGACCACATCGTGCTCGCCGGCG
GCTGCGCGGCCCTGACCGGCCTGCCGGACATGGTGGAAGAGCAGCTGGGCGTGGCCACCGTGGTGGCCAACCCGCTGGCA
CAGATGACCCTGGGCCCGAAGGTGCAGGCACACGCCCTAGCCCAGGACGCCCCGGCGCTGATGATCGCCACCGGTCTGGC
GCTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A498CGW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.852

100

0.509
  comM Acinetobacter nosocomialis M2

50.568

100

0.506
  comM Acinetobacter baylyi ADP1

49.716

100

0.497
  pilM Legionella pneumophila strain ERS1305867

47.009

99.716

0.469

Multiple sequence alignment