Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BJI69_RS04475 Genome accession   NZ_CP017480
Coordinates   960853..961911 (-) Length   352 a.a.
NCBI ID   WP_046966214.1    Uniprot ID   A0A0G9HGS7
Organism   Luteibacter rhizovicinus DSM 16549 strain LJ96     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 955853..966911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJI69_RS04455 (BJI69_04445) - 956679..958904 (-) 2226 WP_046966225.1 type IV pilus secretin PilQ -
  BJI69_RS04460 (BJI69_04450) - 958924..959451 (-) 528 WP_046966211.1 pilus assembly protein PilP -
  BJI69_RS04465 (BJI69_04455) pilO 959451..960098 (-) 648 WP_046966212.1 type 4a pilus biogenesis protein PilO -
  BJI69_RS04470 (BJI69_04460) - 960095..960853 (-) 759 WP_046966213.1 PilN domain-containing protein -
  BJI69_RS04475 (BJI69_04465) pilM 960853..961911 (-) 1059 WP_046966214.1 pilus assembly protein PilM Machinery gene
  BJI69_RS04480 (BJI69_04470) - 962149..964725 (+) 2577 WP_046966215.1 penicillin-binding protein 1A -
  BJI69_RS04485 (BJI69_04475) - 964757..965428 (+) 672 WP_046966216.1 hypothetical protein -
  BJI69_RS04490 (BJI69_04480) - 965473..966768 (-) 1296 WP_046966217.1 citrate synthase -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37895.51 Da        Isoelectric Point: 4.5287

>NTDB_id=198647 BJI69_RS04475 WP_046966214.1 960853..961911(-) (pilM) [Luteibacter rhizovicinus DSM 16549 strain LJ96]
MGLFTPKAAPLIGVDISSTAVKLLQLSQAGGRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRALARSGSKLKHGAAA
VAGSAVITRIIPMSADLSEDDLEGQIQVEANQYIPYPIEEVSLDFEVVGPVRDNPEMNNILLAASRTENVDMRIAALDLG
GLTARVVDVEAFAMENAFAMVADQLAVSRDALVAVVDIGATMTTLAVLKNQRTIYSREQVFGGKQLTDEIMRRYGLSYEE
AGRAKRKGGLPESYEMEALEPFKESLVQQVSRLLQFFFAGSEYSRVDQVVLAGGCASIEGITELLEQQLGVPCVVANPLA
RMALSSRVQAQTLAQDAPALMIAVGLAMRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=198647 BJI69_RS04475 WP_046966214.1 960853..961911(-) (pilM) [Luteibacter rhizovicinus DSM 16549 strain LJ96]
GTGGGGCTCTTTACACCCAAGGCGGCGCCTTTAATTGGCGTCGACATCAGTTCGACCGCAGTAAAGCTGCTGCAGCTCAG
TCAGGCAGGGGGGCGCTATCGCGTCGAACACTATGCCGTCGAGCCGCTGCCGCCCAATGCCGTCGTCGAAAAGAACATCG
TTGAAGTCGAAGCGGTGGGCGAGGCTATTCGGCGGGCATTGGCCCGATCCGGATCCAAACTCAAACACGGCGCTGCTGCC
GTGGCCGGCTCCGCCGTGATCACCCGCATCATTCCCATGTCGGCCGACCTCTCGGAGGATGACCTCGAGGGTCAGATCCA
GGTCGAGGCGAACCAGTACATTCCCTACCCGATCGAGGAAGTTAGCCTCGATTTCGAGGTTGTCGGGCCGGTTCGCGACA
ATCCCGAGATGAACAACATCCTGCTCGCCGCCTCGCGGACCGAGAACGTGGACATGCGCATCGCCGCCCTCGACCTCGGC
GGCTTGACCGCCCGGGTAGTCGACGTCGAGGCGTTCGCCATGGAGAACGCGTTCGCCATGGTGGCCGACCAGTTGGCTGT
CTCCCGGGACGCCCTGGTGGCGGTCGTCGATATCGGCGCCACCATGACGACCCTGGCCGTGCTGAAGAACCAGCGGACGA
TCTACTCTCGCGAGCAGGTCTTCGGCGGCAAGCAGCTCACCGACGAGATCATGCGTCGCTATGGCCTTTCCTACGAGGAA
GCCGGTCGGGCCAAGCGCAAGGGCGGCCTGCCCGAATCCTACGAGATGGAGGCGCTGGAGCCGTTCAAGGAGTCGCTGGT
CCAGCAGGTGAGTCGTCTGCTCCAGTTCTTCTTTGCAGGCAGCGAGTACAGCCGCGTCGACCAGGTCGTCCTGGCCGGTG
GTTGTGCCTCGATCGAAGGCATCACCGAACTGCTAGAACAGCAGCTCGGCGTGCCCTGCGTCGTCGCCAATCCGCTCGCC
CGCATGGCGCTTTCCAGTCGTGTCCAGGCCCAGACCCTGGCCCAGGACGCGCCCGCGCTGATGATTGCCGTCGGCCTCGC
CATGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G9HGS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

52.339

97.159

0.509
  comM Acinetobacter nosocomialis M2

52.047

97.159

0.506
  comM Acinetobacter baylyi ADP1

51.297

98.58

0.506
  pilM Legionella pneumophila strain ERS1305867

41.36

100

0.415

Multiple sequence alignment