Detailed information NTG0197980

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BI364_RS00955
• Genome accession: NZ_CP017415
• Coordinates: 194731..195792 (-)
• Length: 353 a.a.
• NCBI ID: WP_070077162.1
• Uniprot ID: A0A1D8IJX4
• Organism: Acidihalobacter yilgarnensis strain F5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 189731..200792

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BI364_RS00930 (BI364_00930)	aroK	190178..190699 (-)	522	WP_070077158.1	shikimate kinase AroK	-
BI364_RS00935 (BI364_00935)	pilQ	190752..192824 (-)	2073	WP_070077159.1	type IV pilus secretin PilQ	-
BI364_RS00940 (BI364_00940)	-	192956..193531 (-)	576	WP_083251080.1	pilus assembly protein PilP	-
BI364_RS00945 (BI364_00945)	-	193533..194150 (-)	618	WP_070077160.1	type 4a pilus biogenesis protein PilO	-
BI364_RS00950 (BI364_00950)	-	194153..194728 (-)	576	WP_070077161.1	PilN domain-containing protein	-
BI364_RS00955 (BI364_00955)	pilM	194731..195792 (-)	1062	WP_070077162.1	pilus assembly protein PilM	Machinery gene
BI364_RS00960 (BI364_00960)	-	195971..198532 (+)	2562	WP_070077163.1	penicillin-binding protein 1A	-
BI364_RS00965 (BI364_00965)	-	198655..199263 (+)	609	WP_070077164.1	hypothetical protein	-
BI364_RS00970 (BI364_00970)	hemE	199260..200330 (-)	1071	WP_070077165.1	uroporphyrinogen decarboxylase	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 37848.04 Da
• Isoelectric Point: 4.4653

>NTDB_id=197980 BI364_RS00955 WP_070077162.1 194731..195792(-) (pilM) [Acidihalobacter yilgarnensis strain F5]
MSLLKPKKQPLLGIDISSTAVKLVELSRHGTDYRVESYAVEPLPMNAVTEKNVADVDAVGDALRRAVKKAGTSNKFCALA
VPSSAVISKTITLPATLKDSELEGQIEIEADQYIPYSLEEINLDFEVLGPSPGNPDMLEVLLVASRSENVDVRVGAADLA
GLKPKILDVESYATEHAVAQTSGQESQNESAKIVAVIDVGATMTSISVLEDGVITYTREQPFGGKMLTEDIMRRYGLSYE
EAGLAKKEGGLPDNYVAEILEPFKDNMAQQVHRFLQFYYAASEHQSVDHILLAGGCASIAGIDEIIESKLGTPTTIANPF
SRMSIASGVNAQRLGSDAPALMIACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=197980 BI364_RS00955 WP_070077162.1 194731..195792(-) (pilM) [Acidihalobacter yilgarnensis strain F5]
GTGTCGCTCCTTAAACCCAAGAAACAGCCATTGCTGGGGATAGACATCAGTTCGACAGCTGTGAAGCTCGTTGAACTGAG
TCGGCATGGTACCGACTACCGCGTCGAGTCATACGCGGTGGAACCTTTGCCGATGAACGCCGTGACCGAAAAAAACGTGG
CGGACGTCGACGCCGTCGGAGATGCCTTGCGACGCGCCGTCAAGAAGGCCGGCACCAGCAACAAGTTCTGCGCCCTCGCC
GTCCCCTCCTCTGCCGTCATCAGCAAGACAATCACGCTTCCTGCTACGCTCAAGGATTCCGAGCTTGAAGGTCAGATCGA
AATCGAGGCCGACCAGTACATCCCCTACTCCCTGGAAGAGATTAATCTGGATTTCGAGGTCCTCGGCCCGTCCCCCGGCA
ACCCCGATATGCTGGAGGTTCTCCTCGTCGCCTCGCGCAGCGAAAATGTCGATGTGCGCGTCGGAGCGGCCGACCTGGCC
GGCCTAAAACCCAAAATCCTCGACGTCGAATCTTACGCGACGGAGCATGCCGTCGCGCAGACCAGTGGACAAGAATCTCA
GAACGAATCCGCAAAGATCGTCGCCGTCATCGATGTCGGCGCCACCATGACCAGCATCTCGGTTCTCGAAGACGGCGTCA
TTACGTATACGCGGGAACAGCCTTTCGGGGGCAAGATGCTGACCGAAGACATCATGCGTCGATATGGCCTGTCGTACGAG
GAAGCCGGCCTGGCAAAGAAAGAGGGTGGCTTGCCCGACAATTATGTGGCCGAGATCCTCGAACCCTTCAAGGACAACAT
GGCGCAGCAGGTTCACCGATTCCTTCAATTCTACTATGCCGCCAGCGAGCACCAATCGGTCGATCACATTCTCTTGGCTG
GCGGCTGCGCTTCCATCGCCGGAATCGATGAAATCATCGAGTCCAAGCTCGGCACCCCCACCACCATCGCCAACCCGTTC
AGCCGAATGTCGATCGCCTCGGGCGTGAATGCACAGCGCCTGGGCAGCGACGCACCGGCACTTATGATCGCGTGTGGACT
GGCCCTGAGGAGCTTCGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

3D structure

• AlphaFold DB: A0A1D8IJX4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.289	98.867	0.507
comM	Acinetobacter nosocomialis M2	51.289	98.867	0.507
pilM	Legionella pneumophila strain ERS1305867	48.159	100	0.482
comM	Acinetobacter baylyi ADP1	47.025	100	0.47