Detailed information NTG0197508

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AXX03_RS27220
• Genome accession: NZ_CP017306
• Coordinates: 5739829..5740893 (-)
• Length: 354 a.a.
• NCBI ID: WP_096834204.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain PA_150577
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5734829..5745893

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AXX03_RS27195 (AXX03_26380)	aroK	5735364..5735882 (-)	519	WP_003095833.1	shikimate kinase AroK	-
AXX03_RS27200 (AXX03_26385)	pilQ	5735894..5738038 (-)	2145	WP_014603377.1	type 4a pilus secretin PilQ	Machinery gene
AXX03_RS27205 (AXX03_26390)	pilP	5738092..5738616 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	-
AXX03_RS27210 (AXX03_26395)	pilO	5738613..5739236 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	-
AXX03_RS27215 (AXX03_26400)	pilN	5739233..5739829 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	-
AXX03_RS27220 (AXX03_26405)	pilM	5739829..5740893 (-)	1065	WP_096834204.1	type IV pilus assembly protein PilM	Machinery gene
AXX03_RS27225 (AXX03_26410)	-	5741078..5743546 (+)	2469	WP_003114576.1	penicillin-binding protein 1A	-
AXX03_RS27230 (AXX03_26415)	-	5743651..5744919 (-)	1269	WP_004347579.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38112.64 Da
• Isoelectric Point: 4.5405

>NTDB_id=197508 AXX03_RS27220 WP_096834204.1 5739829..5740893(-) (pilM) [Pseudomonas aeruginosa strain PA_150577]
MLGLIKKKANTLLWIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=197508 AXX03_RS27220 WP_096834204.1 5739829..5740893(-) (pilM) [Pseudomonas aeruginosa strain PA_150577]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGTGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (15-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.618	100	0.559
comM	Acinetobacter nosocomialis M2	55.618	100	0.559
comM	Acinetobacter baylyi ADP1	51.695	100	0.517
pilM	Legionella pneumophila strain ERS1305867	46.328	100	0.463