Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   BB164_RS01045 Genome accession   NZ_CP021867
Coordinates   173002..174045 (+) Length   347 a.a.
NCBI ID   WP_223295889.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain SG-M25     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 168002..179045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BB164_RS01030 (BB164_01030) - 171599..171964 (+) 366 WP_000285373.1 DUF1033 family protein -
  BB164_RS01040 (BB164_01040) comGA 172137..173108 (+) 972 WP_000250805.1 competence type IV pilus ATPase ComGA Machinery gene
  BB164_RS01045 (BB164_01045) comGB 173002..174045 (+) 1044 WP_223295889.1 competence type IV pilus assembly protein ComGB Machinery gene
  BB164_RS01050 (BB164_01050) - 174042..174110 (+) 69 Protein_150 competence protein ComGF -
  BB164_RS01055 (BB164_01055) - 174119..175573 (-) 1455 WP_001044789.1 recombinase family protein -
  BB164_RS01060 (BB164_01060) - 175731..176069 (-) 339 WP_000390807.1 hypothetical protein -
  BB164_RS01065 (BB164_01065) - 176073..176501 (-) 429 WP_000200806.1 hypothetical protein -
  BB164_RS01070 (BB164_01070) - 176513..177097 (-) 585 WP_000823130.1 hypothetical protein -
  BB164_RS01075 (BB164_01075) - 177100..177297 (-) 198 WP_000880827.1 hypothetical protein -
  BB164_RS01080 (BB164_01080) - 177316..178056 (-) 741 WP_000580170.1 XRE family transcriptional regulator -
  BB164_RS01085 (BB164_01085) - 178210..178431 (+) 222 WP_000215930.1 helix-turn-helix transcriptional regulator -
  BB164_RS11815 - 178686..178835 (+) 150 WP_001074679.1 BOW99_gp33 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39936.65 Da        Isoelectric Point: 9.9002

>NTDB_id=197283 BB164_RS01045 WP_223295889.1 173002..174045(+) (comGB) [Streptococcus agalactiae strain SG-M25]
MDKWISWLKKDISVRNRHKSKKLSLKKQRKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDSMNKALLEGKDLSKML
GELGFSDTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGLRNYLMPQLGENNFATR
LITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTIPLVGSYVKLYLTAYYAREWGNLLSQGIELDQIVKVMQN
QKSKLFREIGYDMEEGFLSGKAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQPVI
FIFVALIIVMIYAAMLLPMYQNMEILS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=197283 BB164_RS01045 WP_223295889.1 173002..174045(+) (comGB) [Streptococcus agalactiae strain SG-M25]
ATAGACAAGTGGATATCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAA
ACAACGGAAAGTAGTCCAACTTTTTAATAATCTTTTTGCTAGTGGTTTTTCTTTAACTGATATGGTCACCTTCTTAAAGA
GGAGTAAGTTATTGTCTGATTGTTATACAGATAGTATGAATAAGGCATTATTAGAGGGAAAAGATTTATCAAAAATGTTA
GGAGAGTTAGGTTTTTCTGACACTGTTATCACACAGGTTGCATTAGCTGATTTGCATGGTAACATTTCAAGGAGCCTACT
AAAGATTGAGTCTTATTTAGCTAATCTTTTGTTAGTTAGAAAAAAAGTAATAGAAGTAGCTACTTACCCATTGATATTAT
TGTCTTTTCTGGTGCTAATTATGATTGGCCTTAGGAATTATTTAATGCCCCAATTAGGAGAAAATAATTTTGCAACTAGA
CTGATTACAAATGTGCCGAATATTTTCTTATTACTTTTAGCAGTTGTACTTATTTTTAGTTTAATATTTTATATTATTCA
AAAGCGATTGTCGCGCATTAAAGTAGCTTGTTTTTTAACAACAATTCCCTTAGTTGGATCATATGTTAAGCTTTATTTAA
CTGCTTACTATGCCCGTGAGTGGGGAAATTTATTAAGTCAAGGTATTGAATTGGACCAAATTGTTAAAGTAATGCAAAAT
CAGAAATCCAAACTTTTTAGGGAAATAGGATATGACATGGAAGAAGGTTTTCTATCAGGTAAAGCATTTCACCAAAAAGT
ATTAGACTATCCGTTTTTCTTAACTGAGCTTAGTTTAATGATTGAATATGGCCAAGTTAAGGCGAAATTAGGAACAGAGT
TAGATATATATGCTGATGAGAAGTGGGAGGATTTTTTTACAAAATTAGCTAGAGCGACCCAGTTAATCCAACCCGTTATT
TTTATTTTTGTAGCTCTTATCATTGTTATGATTTATGCAGCAATGCTGTTACCAATGTATCAAAATATGGAGATATTATC
ATGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(34-150)

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Streptococcus mutans UA140

62.17

98.271

0.611
  comGB Streptococcus mutans UA159

61.877

98.271

0.608
  comGB Streptococcus gordonii str. Challis substr. CH1

56.305

98.271

0.553
  comGB/cglB Streptococcus mitis NCTC 12261

55.224

96.542

0.533
  comGB/cglB Streptococcus mitis SK321

54.627

96.542

0.527
  comGB/cglB Streptococcus pneumoniae Rx1

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae D39

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae R6

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae TIGR4

53.731

96.542

0.519
  comGB Lactococcus lactis subsp. cremoris KW2

50.148

97.118

0.487