Detailed information NTG0194425

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PsaNZ45_RS28825
• Genome accession: NZ_CP017007
• Coordinates: 5950503..5951567 (-)
• Length: 354 a.a.
• NCBI ID: WP_003378852.1
• Uniprot ID: A0A2G9KZJ9
• Organism: Pseudomonas syringae pv. actinidiae strain ICMP 20586
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5945503..5956567

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PsaNZ45_RS28800 (PsaNZ45_26745)	aroK	5946057..5946575 (-)	519	WP_003378847.1	shikimate kinase AroK	-
PsaNZ45_RS28805 (PsaNZ45_26750)	pilQ	5946580..5948685 (-)	2106	WP_017682642.1	type IV pilus secretin PilQ	Machinery gene
PsaNZ45_RS28810 (PsaNZ45_26755)	pilP	5948733..5949260 (-)	528	WP_003378849.1	type 4a pilus biogenesis lipoprotein PilP	-
PsaNZ45_RS28815 (PsaNZ45_26760)	pilO	5949257..5949880 (-)	624	WP_003378850.1	type 4a pilus biogenesis protein PilO	-
PsaNZ45_RS28820 (PsaNZ45_26765)	-	5949877..5950503 (-)	627	WP_003378851.1	PilN domain-containing protein	-
PsaNZ45_RS28825 (PsaNZ45_26770)	pilM	5950503..5951567 (-)	1065	WP_003378852.1	pilus assembly protein PilM	Machinery gene
PsaNZ45_RS28830 (PsaNZ45_26775)	-	5951777..5954218 (+)	2442	WP_370445385.1	penicillin-binding protein 1A	-
PsaNZ45_RS28835 (PsaNZ45_26780)	-	5954544..5955812 (-)	1269	WP_003378855.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38103.62 Da
• Isoelectric Point: 4.7008

>NTDB_id=194425 PsaNZ45_RS28825 WP_003378852.1 5950503..5951567(-) (pilM) [Pseudomonas syringae pv. actinidiae strain ICMP 20586]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=194425 PsaNZ45_RS28825 WP_003378852.1 5950503..5951567(-) (pilM) [Pseudomonas syringae pv. actinidiae strain ICMP 20586]
GTGTTCGAACTCTTTGGCAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCTCGCGTACTGGTCAAGGCCAAGACCAACGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCTGTGATCACCAAAACCATCGAGATGGACGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
TAAGCTTGAAGCTGATCAGTACATCCCTTATCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGTTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAGGCTGCGCTGGCTCTC
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGGCTTCTGGCTGCGCAACTGGG
CAACGGTCATGACGAACTGACCGTTGCCGTGGTAGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCACGGCC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGCCAGCTGACTGACGAAATTCAGCGTCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAAGTGCTGGACCCCTTCAAGGACGC
GCTCGTGCAACAGGTTTCCCGATCCCTGCAGTTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACGCCGACCATGGTTGCCAACCCG
TTTGCCGACATGGCCTTGAGCTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCGCCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A2G9KZJ9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469