Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   S101267_RS14065 Genome accession   NZ_CP021505
Coordinates   2703701..2704705 (-) Length   334 a.a.
NCBI ID   WP_013353043.1    Uniprot ID   A0A9P1JIP2
Organism   Bacillus amyloliquefaciens strain SRCM101267     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2698701..2709705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  S101267_RS14035 (S101267_02816) - 2699727..2700383 (-) 657 WP_013353038.1 DUF421 domain-containing protein -
  S101267_RS14040 (S101267_02817) - 2700550..2700936 (+) 387 WP_013353039.1 TIGR04086 family membrane protein -
  S101267_RS14045 (S101267_02818) yajC 2700989..2701249 (-) 261 WP_003152695.1 preprotein translocase subunit YajC -
  S101267_RS14050 (S101267_02819) tgt 2701281..2702426 (-) 1146 WP_013353040.1 tRNA guanosine(34) transglycosylase Tgt -
  S101267_RS14055 (S101267_02820) queA 2702454..2703482 (-) 1029 WP_013353041.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  S101267_RS14060 (S101267_02821) - 2703508..2703708 (-) 201 WP_013353042.1 DUF2905 domain-containing protein -
  S101267_RS14065 (S101267_02822) ruvB 2703701..2704705 (-) 1005 WP_013353043.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  S101267_RS14070 (S101267_02823) ruvA 2704716..2705321 (-) 606 WP_003152683.1 Holliday junction branch migration protein RuvA Machinery gene
  S101267_RS14075 (S101267_02824) - 2705456..2705932 (-) 477 WP_038463270.1 intercompartmental signaling factor BofC -
  S101267_RS22365 (S101267_02825) - 2706098..2706217 (-) 120 WP_014470775.1 hypothetical protein -
  S101267_RS14080 (S101267_02826) - 2706343..2706908 (-) 566 Protein_2734 YhcN/YlaJ family sporulation lipoprotein -
  S101267_RS14085 (S101267_02827) safA 2707057..2708208 (-) 1152 WP_013353047.1 spore coat assembly protein SafA -
  S101267_RS14090 (S101267_02828) nadA 2708335..2709438 (-) 1104 WP_013353048.1 quinolinate synthase NadA -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37200.48 Da        Isoelectric Point: 4.6703

>NTDB_id=193543 S101267_RS14065 WP_013353043.1 2703701..2704705(-) (ruvB) [Bacillus amyloliquefaciens strain SRCM101267]
MDERLVSSEADSHESIIEQSLRPQNLAQYIGQQKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
MRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLNRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVLSRLEYYTQEELTDIVSRTAEVFEVEIEEAPALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEEIAHDALERLQVDALGLDHIDHKLLMGMIEKFSGGPVGLDTISATIGEEPHTIEDVYEPYLLQIGFLQRTPRGRVVTP
AVYEHFRLEAPACD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=193543 S101267_RS14065 WP_013353043.1 2703701..2704705(-) (ruvB) [Bacillus amyloliquefaciens strain SRCM101267]
ATGGATGAACGGCTCGTTTCAAGTGAAGCAGACAGCCATGAATCAATAATCGAGCAAAGCCTCAGGCCGCAGAACCTCGC
CCAGTACATCGGGCAGCAGAAGGTAAAAGAAAATCTGCGCGTGTTTATCGATGCGGCGAAAATGAGGCAGGAAACGCTTG
ACCACGTTCTTTTATACGGGCCTCCGGGTCTCGGAAAAACAACGCTCGCGTCTATCGTGGCCAATGAGATGGGTGTGGAG
ATGCGGACGACGTCAGGCCCCGCTATCGAAAGACCGGGAGACTTGGCAGCCATTCTGACGGCGCTCGAACCCGGGGATGT
GCTGTTTATAGACGAAATTCACAGACTGAACCGGTCAATCGAAGAAGTGCTGTACCCGGCGATGGAGGACTTCTGCCTTG
ATATCGTTATTGGAAAAGGCCCGTCCGCCCGCTCCGTCCGCCTTGACCTTCCGCCTTTTACGCTGGTAGGAGCGACGACC
AGGGTCGGCTTATTGACCGCGCCGCTCAGAGACCGGTTCGGGGTGCTGTCCCGCCTTGAATACTATACGCAGGAAGAACT
GACAGATATCGTGTCAAGAACGGCTGAAGTATTCGAAGTTGAAATCGAAGAAGCCCCGGCTTTGGAAATCGCCAGACGGT
CAAGGGGAACGCCGCGGGTTGCAAACCGGCTTCTCAGAAGGGTGAGAGATTTTGCCCAAGTGCTAGGTGACAGCCGCATC
ACGGAAGAAATCGCTCACGACGCGCTCGAACGTCTTCAAGTCGACGCACTCGGCCTTGACCATATTGACCATAAGCTGCT
GATGGGCATGATTGAAAAATTCAGCGGCGGTCCCGTCGGCCTTGATACGATTTCCGCGACAATCGGTGAAGAACCGCATA
CAATAGAGGATGTGTACGAACCGTATCTGCTTCAGATCGGATTTCTCCAAAGGACGCCGAGAGGACGGGTCGTGACCCCG
GCTGTGTATGAGCATTTTCGATTGGAGGCACCCGCCTGTGACTGA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)

RuvB AAA lid domain (AAA_lid_4)

(182-255)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A9P1JIP2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

91.916

100

0.919
  ruvB Streptococcus pneumoniae TIGR4

60.736

97.605

0.593
  ruvB Streptococcus pneumoniae R6

60.736

97.605

0.593
  ruvB Streptococcus pneumoniae D39

60.736

97.605

0.593
  ruvB Synechocystis sp. PCC 6803

58.359

98.503

0.575
  ruvB Helicobacter pylori 26695

52.439

98.204

0.515