Detailed information NTG0193069

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BE842_RS00705
• Genome accession: NZ_CP016870
• Coordinates: 143834..144898 (+)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila subsp. pneumophila strain FFI103
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 138834..149898

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BE842_RS00695 (BE842_00695)	-	139874..142258 (-)	2385	WP_011215092.1	penicillin-binding protein 1A	-
BE842_RS00700 (BE842_00700)	ravI	142410..143417 (-)	1008	WP_011215093.1	Dot/Icm T4SS effector RavI	-
BE842_RS00705 (BE842_00705)	pilM	143834..144898 (+)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
BE842_RS00710 (BE842_00710)	pilN	144902..145450 (+)	549	WP_011215095.1	PilN domain-containing protein	Machinery gene
BE842_RS00715 (BE842_00715)	pilO	145464..146063 (+)	600	WP_011215096.1	type 4a pilus biogenesis protein PilO	Machinery gene
BE842_RS00720 (BE842_00720)	pilP	146060..146644 (+)	585	WP_011215097.1	pilus assembly protein PilP	Machinery gene
BE842_RS00725 (BE842_00725)	pilQ	146648..148744 (+)	2097	WP_070322471.1	type IV pilus secretin PilQ	Machinery gene
BE842_RS00730 (BE842_00730)	aroK	149190..149717 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=193069 BE842_RS00705 WP_011215094.1 143834..144898(+) (pilM) [Legionella pneumophila subsp. pneumophila strain FFI103]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=193069 BE842_RS00705 WP_011215094.1 143834..144898(+) (pilM) [Legionella pneumophila subsp. pneumophila strain FFI103]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAAGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTGGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTATCCCATAGATGAAATAAATCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
GGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACCCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCGATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGATAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCATGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407