Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   BC359_RS05275 Genome accession   NZ_CP016790
Coordinates   1019670..1019981 (+) Length   103 a.a.
NCBI ID   WP_025911556.1    Uniprot ID   -
Organism   Priestia flexa strain KLBMP 4941     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1014670..1024981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BC359_RS05245 (BC359_05235) - 1015393..1015566 (-) 174 WP_025911564.1 DUF2759 domain-containing protein -
  BC359_RS05250 (BC359_05240) - 1015763..1016395 (+) 633 WP_025911563.1 MBL fold metallo-hydrolase -
  BC359_RS05255 (BC359_05245) - 1016439..1016678 (-) 240 WP_025911561.1 DUF2626 domain-containing protein -
  BC359_RS05260 (BC359_05250) - 1016958..1017341 (+) 384 WP_061784685.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BC359_RS05265 (BC359_05255) comGA 1017559..1018617 (+) 1059 WP_078989280.1 competence type IV pilus ATPase ComGA Machinery gene
  BC359_RS05270 (BC359_05260) comGB 1018598..1019656 (+) 1059 WP_025911558.1 competence type IV pilus assembly protein ComGB -
  BC359_RS05275 (BC359_05265) comGC 1019670..1019981 (+) 312 WP_025911556.1 competence type IV pilus major pilin ComGC Machinery gene
  BC359_RS05280 (BC359_05270) comGD 1019968..1020417 (+) 450 WP_237072035.1 competence type IV pilus minor pilin ComGD -
  BC359_RS05285 (BC359_05275) - 1020401..1020736 (+) 336 WP_025911553.1 type II secretion system protein -
  BC359_RS05290 (BC359_05280) comGF 1020733..1021161 (+) 429 WP_025911552.1 competence type IV pilus minor pilin ComGF -
  BC359_RS05295 (BC359_05285) comGG 1021124..1021543 (+) 420 WP_061784683.1 competence type IV pilus minor pilin ComGG -
  BC359_RS05300 (BC359_05290) - 1021594..1022220 (-) 627 Protein_993 cupin domain-containing protein -
  BC359_RS05305 (BC359_05295) - 1022365..1022556 (+) 192 WP_025911548.1 YqzE family protein -
  BC359_RS05310 (BC359_05300) - 1022594..1023415 (-) 822 WP_076512140.1 YqhG family protein -

Sequence

Protein


Download         Length: 103 a.a.        Molecular weight: 11017.88 Da        Isoelectric Point: 5.7969

>NTDB_id=192263 BC359_RS05275 WP_025911556.1 1019670..1019981(+) (comGC) [Priestia flexa strain KLBMP 4941]
MLKNEKGFTLIEMLIVMLVITVLLLIMVPNVLKHNAVINNKGCSALVKTVEAQVQVYELENGTKPTLNELESGGYLKEGT
VCPNGSKITIGTDGRVSASDGNS

Nucleotide


Download         Length: 312 bp        

>NTDB_id=192263 BC359_RS05275 WP_025911556.1 1019670..1019981(+) (comGC) [Priestia flexa strain KLBMP 4941]
ATGTTAAAAAATGAGAAAGGTTTTACTTTAATTGAGATGCTCATTGTTATGTTAGTTATTACAGTGCTATTGCTTATTAT
GGTGCCAAATGTATTAAAGCATAACGCTGTTATCAACAATAAAGGATGCTCGGCTTTAGTGAAAACAGTGGAAGCACAAG
TGCAGGTGTATGAGCTTGAAAACGGAACAAAGCCTACTTTAAACGAGCTTGAAAGCGGAGGATACTTAAAAGAAGGAACG
GTGTGTCCAAATGGTTCGAAAATTACAATAGGTACGGATGGACGCGTATCAGCGAGTGATGGAAACTCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

53.684

92.233

0.495
  comGC Lactococcus lactis subsp. cremoris KW2

47.5

77.67

0.369
  comGC Staphylococcus aureus MW2

40.86

90.291

0.369
  comGC Staphylococcus aureus N315

40.86

90.291

0.369

Multiple sequence alignment