Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BCV67_RS05235 Genome accession   NZ_CP016756
Coordinates   1223056..1224114 (-) Length   352 a.a.
NCBI ID   WP_057629032.1    Uniprot ID   A0A126NGQ3
Organism   Stenotrophomonas nitritireducens strain 2001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1218056..1229114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCV67_RS05215 - 1219161..1221059 (-) 1899 WP_062166774.1 type IV pilus secretin PilQ family protein -
  BCV67_RS05220 - 1221079..1221609 (-) 531 WP_062166775.1 pilus assembly protein PilP -
  BCV67_RS05225 pilO 1221612..1222277 (-) 666 WP_062166776.1 type 4a pilus biogenesis protein PilO -
  BCV67_RS05230 - 1222274..1223056 (-) 783 WP_062166777.1 PilN domain-containing protein -
  BCV67_RS05235 pilM 1223056..1224114 (-) 1059 WP_057629032.1 pilus assembly protein PilM Machinery gene
  BCV67_RS05240 - 1224330..1226762 (+) 2433 WP_062166778.1 penicillin-binding protein 1A -
  BCV67_RS05245 - 1226836..1227462 (+) 627 WP_062166779.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37435.90 Da        Isoelectric Point: 4.4273

>NTDB_id=191901 BCV67_RS05235 WP_057629032.1 1223056..1224114(-) (pilM) [Stenotrophomonas nitritireducens strain 2001]
MGLIPKSQTPIIGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAVARSGSKAKGAAAA
VAGSAVITKVIPMPADLDENDLEAQVELEAVNYIPYPIDEVNLDFEVLGPVPNNPEMVQVLLAASRSENVELRQSALELG
GLTAKVMDVEAFAIENAFALVASELAVPSDGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYEVEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAALAGLPTMVEEQLGVATSVANPLA
QMTLGPKVQAHALNQDAPALMIATGLAMRGFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=191901 BCV67_RS05235 WP_057629032.1 1223056..1224114(-) (pilM) [Stenotrophomonas nitritireducens strain 2001]
GTGGGGCTTATCCCAAAGAGTCAGACGCCCATCATTGGCGTCGACATCAGTTCGACTGCGGTAAAGCTGTTGCAGCTTTC
CCGCAGCGGTAATCGTTTTCGCGTGGAACATTACGCCGTGGAACCTCTTCCGCCGAATGCGGTGGTGGAGAAGAACATTG
TCGAAGTCGAGGCAGTCGGCGAAGCGATTCGTCGGGCTGTTGCGCGTTCAGGCAGCAAGGCCAAGGGGGCTGCTGCTGCT
GTTGCCGGTTCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGACCTGGACGAGAACGACCTGGAGGCACAGGTCGA
GCTGGAGGCGGTGAACTACATCCCGTACCCCATCGACGAAGTAAACCTCGATTTCGAAGTGCTGGGTCCAGTGCCGAACA
ATCCAGAAATGGTGCAGGTACTGCTGGCCGCTTCGCGTTCGGAAAACGTCGAGCTGCGCCAGTCCGCGTTGGAGCTGGGT
GGGCTGACCGCCAAGGTGATGGATGTGGAGGCGTTCGCGATTGAGAACGCATTCGCATTGGTCGCCAGCGAGCTGGCTGT
ACCCAGTGATGGCGTGGTGGCGCTGGTCGACATCGGTGCAACCATGACCACGCTCAACGTGTTGCGCGGTGGTCGCAGCC
TGTACAGCCGCGAACAGGTGTTCGGCGGCAAACAGCTCACCGACGAGGTGATGCGCCGTTACGGGCTCAGCTACGAGGAA
GCAGGCCTGGCCAAGCGCCAGGGCGGCTTGCCGGAAAGCTATGAGGTTGAAGTGCTGGAGCCGTTCAAGGAGGCGACAGT
GCAGCAGATCAGCCGCCTGTTGCAGTTCTTCTACGCCGGCAGCGAGTTCAACCGGGTCGACCACATCGTGTTGGCGGGCG
GTTGTGCCGCTCTGGCCGGTTTGCCGACCATGGTTGAAGAACAGCTGGGTGTAGCCACATCGGTAGCCAATCCCTTGGCA
CAGATGACGCTGGGACCCAAGGTCCAGGCGCATGCACTGAACCAGGATGCACCGGCGCTGATGATCGCGACGGGTCTGGC
CATGAGGGGGTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A126NGQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

49.716

100

0.497
  comM Acinetobacter nosocomialis M2

49.716

100

0.497
  comM Acinetobacter baylyi ADP1

49.432

100

0.494
  pilM Legionella pneumophila strain ERS1305867

47.989

98.864

0.474

Multiple sequence alignment