Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BC375_RS01630 Genome accession   NZ_CP016610
Coordinates   374198..375256 (+) Length   352 a.a.
NCBI ID   WP_038200243.1    Uniprot ID   -
Organism   Xylella fastidiosa strain Salento-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 369198..380256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BC375_RS01620 (BC375_01530) - 370259..371224 (+) 966 WP_023907274.1 aldo/keto reductase -
  BC375_RS01625 (BC375_01535) - 371527..373956 (-) 2430 WP_046417965.1 penicillin-binding protein 1A -
  BC375_RS01630 (BC375_01540) pilM 374198..375256 (+) 1059 WP_038200243.1 type IV pilus biogenesis protein PilM Machinery gene
  BC375_RS01635 (BC375_01545) - 375256..375816 (+) 561 Protein_294 PilN domain-containing protein -
  BC375_RS01640 (BC375_01550) - 375918..376590 (+) 673 Protein_295 type 4a pilus biogenesis protein PilO -
  BC375_RS01645 (BC375_01555) - 376587..377117 (+) 531 WP_023907278.1 pilus assembly protein PilP -
  BC375_RS01650 (BC375_01560) - 377138..379030 (+) 1893 WP_171817040.1 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37615.25 Da        Isoelectric Point: 4.6541

>NTDB_id=189662 BC375_RS01630 WP_038200243.1 374198..375256(+) (pilM) [Xylella fastidiosa strain Salento-2]
MGLFSKKQSVLVGVDISSTAVKLLQLSRSGNRFKVEHYAVEPLPLNAVAERGIVEVEQVGEAIRRAVSRSGTKAKFAAAA
VAGSAVITKLIPMPVGLDEQDLEAQIEIEATNYIPYPIEEVSLDFEVLGPVPNNTEMVQVLLAASRSENVELRQSALELG
GLTAKVIDVEALAVENAFSLIAQELSVGSNALVALIDIGATMSTLNVLHSGRSLYTREQLFGGKQLTDEVMHRYGMTYEE
AGQAKRQGGLPQSYEVEVLGSFKDSVIQQISRLLQFFYAGSEYNRVDCIVLAGGCAVIAGLPAMVEERLGVVTVVANPLA
QMTLGAKVQAHTLAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=189662 BC375_RS01630 WP_038200243.1 374198..375256(+) (pilM) [Xylella fastidiosa strain Salento-2]
GTGGGGCTTTTTTCCAAAAAACAGTCGGTATTGGTTGGTGTCGACATTAGTTCAACTGCAGTGAAGCTCTTACAGCTTTC
CCGCAGTGGTAATCGCTTCAAGGTGGAACACTATGCTGTGGAGCCGCTACCTCTGAATGCGGTCGCCGAGAGGGGCATCG
TTGAAGTCGAGCAGGTTGGTGAAGCAATCCGTCGTGCGGTGAGTCGTTCTGGAACTAAGGCCAAATTTGCCGCTGCTGCG
GTCGCCGGTTCGGCAGTGATCACCAAGTTGATACCGATGCCTGTTGGTCTGGACGAGCAGGATTTGGAAGCCCAGATAGA
AATAGAGGCTACGAATTACATCCCGTACCCTATTGAAGAGGTCAGCCTAGATTTCGAGGTGCTTGGTCCGGTTCCCAACA
ACACGGAGATGGTCCAGGTTTTGCTTGCTGCGTCTCGTTCAGAGAATGTCGAATTGCGCCAATCTGCGTTGGAGTTGGGT
GGGTTGACTGCCAAGGTTATTGACGTGGAGGCTTTAGCGGTTGAGAACGCTTTCTCTCTTATTGCCCAAGAACTGTCGGT
TGGGAGTAATGCATTGGTTGCATTGATCGACATCGGTGCAACGATGTCGACGCTTAATGTGCTGCATAGTGGTCGCAGTT
TGTACACGCGCGAGCAGCTATTTGGTGGTAAGCAGCTTACTGACGAAGTGATGCATCGCTATGGGATGACTTACGAGGAG
GCTGGTCAGGCCAAGCGTCAAGGAGGTTTGCCGCAAAGCTATGAGGTTGAAGTGCTGGGATCATTTAAGGACTCTGTGAT
CCAGCAGATCAGCCGTTTATTACAGTTTTTCTATGCTGGCAGTGAGTACAACCGAGTTGATTGCATCGTGTTGGCGGGAG
GCTGTGCTGTGATTGCGGGTTTGCCGGCTATGGTCGAAGAACGGTTGGGTGTCGTTACCGTTGTGGCTAATCCGCTTGCG
CAGATGACTTTAGGGGCGAAAGTGCAAGCACACACGCTTGCTCAAGATGCACCTGCTTTAATGATTGCTACTGGTCTGGC
TTTGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

48.295

100

0.483
  comM Acinetobacter nosocomialis M2

48.295

100

0.483
  comM Acinetobacter baylyi ADP1

47.443

100

0.474
  pilM Legionella pneumophila strain ERS1305867

45.326

100

0.455

Multiple sequence alignment