Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   A6B42_RS07450 Genome accession   NZ_CP016607
Coordinates   1523459..1524013 (-) Length   184 a.a.
NCBI ID   WP_178218449.1    Uniprot ID   -
Organism   Mannheimia varigena strain F222     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1518459..1529013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6B42_RS07425 (A6B42_07425) - 1518857..1519678 (+) 822 WP_178218441.1 SDR family NAD(P)-dependent oxidoreductase -
  A6B42_RS07430 (A6B42_07430) - 1519693..1520244 (-) 552 WP_178218443.1 hypothetical protein -
  A6B42_RS07435 (A6B42_07435) - 1520228..1520752 (-) 525 WP_178218445.1 hypothetical protein -
  A6B42_RS07440 (A6B42_07440) - 1520763..1521005 (-) 243 WP_025342947.1 hypothetical protein -
  A6B42_RS07445 (A6B42_07445) - 1521134..1523458 (-) 2325 WP_178218447.1 Tex family protein -
  A6B42_RS07450 (A6B42_07450) pilF 1523459..1524013 (-) 555 WP_178218449.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  A6B42_RS07455 (A6B42_07455) - 1524227..1524916 (+) 690 WP_178218451.1 CPBP family intramembrane glutamic endopeptidase -
  A6B42_RS07460 (A6B42_07460) - 1524930..1525076 (+) 147 WP_178216494.1 type II secretion system protein -
  A6B42_RS07465 (A6B42_07465) - 1525122..1525901 (-) 780 WP_178218453.1 MerR family transcriptional regulator -
  A6B42_RS07470 (A6B42_07470) - 1526162..1526479 (-) 318 WP_178218455.1 hypothetical protein -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20872.64 Da        Isoelectric Point: 7.7109

>NTDB_id=189653 A6B42_RS07450 WP_178218449.1 1523459..1524013(-) (pilF) [Mannheimia varigena strain F222]
MFAKYLKNITACITLFLLAACSSHTETASETEVNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDPNDYLPHSVLA
YYYQQTADIQNAEKAYQQALKLSQNRPDVRNNYGTFLCKQGQFQQAYQQFEQAVASPKPYYHQADSLENIILCAKKEPNP
QKVAETLIELEKLDKPRATLLKGN

Nucleotide


Download         Length: 555 bp        

>NTDB_id=189653 A6B42_RS07450 WP_178218449.1 1523459..1524013(-) (pilF) [Mannheimia varigena strain F222]
ATGTTTGCAAAATATTTGAAAAATATCACCGCTTGTATCACGCTATTTTTGTTAGCTGCGTGTTCCTCCCACACCGAAAC
CGCTTCGGAAACTGAAGTTAACCGCTCGGAAGCGGTGAAAGCTCGTATAAACTTAGCCCTTGCTTATTTGGAGCAGAACG
ATTTCCCTAAAGCGAAGCAGAATATTGACAAAGCCCTCGCTCACGACCCGAACGATTACCTGCCCCATTCGGTGCTTGCC
TACTATTATCAGCAAACTGCCGATATACAAAATGCCGAAAAAGCCTATCAACAAGCCTTGAAATTAAGCCAAAACCGCCC
TGATGTGCGTAATAATTACGGCACTTTTTTATGTAAACAAGGTCAATTTCAGCAGGCTTATCAACAATTTGAACAGGCAG
TGGCGAGTCCGAAGCCTTATTATCACCAAGCGGATAGTTTAGAGAACATCATTTTATGTGCGAAAAAAGAGCCTAATCCG
CAAAAAGTTGCAGAAACACTTATTGAGCTTGAGAAACTAGACAAACCTCGAGCCACATTATTGAAAGGAAATTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_1)

(40-72)

Tetratricopeptide repeat (TPR_17)

(95-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.102

100

0.641
  pilF/pilF2 Haemophilus influenzae Rd KW20

48.837

93.478

0.457

Multiple sequence alignment