Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   A6B42_RS06560 Genome accession   NZ_CP016607
Coordinates   1348714..1350027 (+) Length   437 a.a.
NCBI ID   WP_178218206.1    Uniprot ID   -
Organism   Mannheimia varigena strain F222     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1343714..1355027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6B42_RS06535 (A6B42_06535) - 1343879..1346431 (-) 2553 WP_178218197.1 penicillin-binding protein 1A -
  A6B42_RS06540 (A6B42_06540) - 1346557..1347258 (+) 702 WP_178218199.1 competence protein ComA -
  A6B42_RS06545 (A6B42_06545) - 1347234..1347755 (+) 522 WP_178218201.1 hypothetical protein -
  A6B42_RS06550 (A6B42_06550) - 1347757..1348290 (+) 534 WP_178218203.1 hypothetical protein -
  A6B42_RS06555 (A6B42_06555) - 1348292..1348705 (+) 414 WP_178218204.1 hypothetical protein -
  A6B42_RS06560 (A6B42_06560) comE 1348714..1350027 (+) 1314 WP_178218206.1 type IV pilus secretin PilQ Machinery gene
  A6B42_RS06565 (A6B42_06565) nusB 1350107..1350520 (+) 414 WP_112110692.1 transcription antitermination factor NusB -
  A6B42_RS06570 (A6B42_06570) thiL 1350583..1351563 (+) 981 WP_178218208.1 thiamine-phosphate kinase -
  A6B42_RS06575 (A6B42_06575) - 1351569..1352042 (+) 474 WP_178218210.1 phosphatidylglycerophosphatase A -
  A6B42_RS06580 (A6B42_06580) - 1352050..1352676 (+) 627 WP_178218212.1 LysE family transporter -
  A6B42_RS06585 (A6B42_06585) ruvA 1352785..1353390 (+) 606 WP_178218214.1 Holliday junction branch migration protein RuvA -
  A6B42_RS06590 (A6B42_06590) ruvB 1353401..1354405 (+) 1005 WP_178218216.1 Holliday junction branch migration DNA helicase RuvB -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48361.45 Da        Isoelectric Point: 8.9392

>NTDB_id=189649 A6B42_RS06560 WP_178218206.1 1348714..1350027(+) (comE) [Mannheimia varigena strain F222]
MFRIFLYLLLSSSLALADTISLAVKNAPTSLLLTYLAEETGKNIVLDDDIQTKSTLRLENKSINEIFKTLSKVNKLSFTQ
EADIVYISKKEEKSADLNSVSIAHLQNNIQNPPLVTSPKLITKIIKLNYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIIKDSAKSLQNIERLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGMFSRGTDHYKFGGRLEGNGLSNVANNLNVN
FPVANGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNTQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKIPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKVRGVKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=189649 A6B42_RS06560 WP_178218206.1 1348714..1350027(+) (comE) [Mannheimia varigena strain F222]
ATGTTTAGAATTTTTCTGTATTTATTACTTAGTTCGTCATTAGCATTGGCTGACACTATTTCCCTTGCCGTAAAGAATGC
ACCAACTTCACTTCTGCTAACCTATTTAGCTGAAGAAACAGGTAAAAATATAGTATTAGATGATGATATTCAAACTAAAT
CCACCTTAAGATTAGAAAATAAATCCATTAATGAGATTTTTAAAACATTGAGCAAAGTAAATAAATTATCTTTTACTCAA
GAAGCAGACATTGTCTATATTAGTAAAAAAGAAGAAAAATCGGCTGATTTAAATTCAGTTTCAATTGCCCATTTGCAAAA
TAATATACAAAACCCACCGCTTGTAACTTCTCCAAAACTGATAACTAAAATCATAAAACTAAATTATGCTAAAGCCTCAG
AAGTGATTGAATCTTTAACTAAAGGCAGTGGCACGTTTTTATCTGAAAATGGTTATATCCATTTTGACGAGCGAAGTAAT
AGCTTAATTATCAAAGATAGTGCGAAATCACTACAAAATATTGAGCGATTAGTCAAACAACTCGACCAACCCACCGAACA
AATTGCGATTGAAGCTCGCATTGTCACGATTAGTAGCGAACATTTGCAAGAATTAGGTGTGCGTTGGGGAATGTTTTCCA
GAGGAACTGATCACTATAAATTTGGTGGGCGATTGGAAGGAAATGGGTTAAGCAATGTAGCTAATAATCTCAATGTGAAT
TTCCCCGTTGCGAATGGTGCTTCAGCAGTTTTACAAGTAGCTTCCATTAATAGTCGAGTACTAGATTTAGAACTCAGTGC
ATTAGAACAGGAAAACAGTGTGGAAATCATTGCTAGCCCTCGTCTTTTAACCACTAATAAAAAACCTGCGAGTATCAAGC
AAGGCACAGAAATTCCTTATGTAATGTATAACACCAAATCAGAAGCAACCGATGTCGAATTTAAAGAAGCAGTGTTAGGA
TTGGAAGTTGTTCCTCATCTTTCTACTCAAAATCAAATTTTGTTGGATTTAATCGTCACTCAAAACTCACCGAATACACA
ATCTGGTAGCAGTGGTTTAATTACCATTGATAAGCAAGAACTTAATACACAGGTGTTTGCTAAACACGGCGAAACTATCG
TGCTAGGCGGTATTTTCCAACATTTAACCCAAAAAGGAGAAGATAAAATACCTATTTTAGGCTCAATTCCGTTTATTAAA
CGATTATTTAGCCAAACAAGAGACAAAATCAGCAAGCGGGAACTTGTTATTTTTGTTACCCCTTACATTCTCCAATCAAA
CGAAAAAGTAAGGGGCGTGAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)

Bacterial type II and III secretion system protein (Secretin)

(267-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

62.759

99.542

0.625
  comE Haemophilus influenzae Rd KW20

50.605

94.508

0.478
  comE Haemophilus influenzae 86-028NP

49.879

94.508

0.471
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.094

97.025

0.389
  pilQ Vibrio cholerae strain A1552

40.094

97.025

0.389
  pilQ Vibrio campbellii strain DS40M4

38.283

98.627

0.378

Multiple sequence alignment