Detailed information    

insolico Bioinformatically predicted

Overview


Name   comYF   Type   Machinery gene
Locus tag   BBD27_RS05185 Genome accession   NZ_CP016394
Coordinates   970936..971373 (+) Length   145 a.a.
NCBI ID   WP_011681686.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain ND07     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 965936..976373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBD27_RS05155 (BBD27_1006) - 967610..967972 (+) 363 WP_014622006.1 DUF1033 family protein -
  BBD27_RS05160 (BBD27_1007) comGA/cglA/cilD 968053..968994 (+) 942 WP_022096896.1 competence type IV pilus ATPase ComGA Machinery gene
  BBD27_RS05165 (BBD27_1008) comYB 968876..969976 (+) 1101 WP_120764773.1 competence type IV pilus assembly protein ComGB Machinery gene
  BBD27_RS05170 (BBD27_1009) comYC 969973..970299 (+) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  BBD27_RS05175 (BBD27_1010) comYD 970259..970687 (+) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  BBD27_RS05180 comGE 970659..970952 (+) 294 WP_024704238.1 competence type IV pilus minor pilin ComGE -
  BBD27_RS05185 (BBD27_1012) comYF 970936..971373 (+) 438 WP_011681686.1 competence type IV pilus minor pilin ComGF Machinery gene
  BBD27_RS05190 (BBD27_1013) comGG 971351..971668 (+) 318 WP_011681685.1 competence type IV pilus minor pilin ComGG -
  BBD27_RS05195 (BBD27_1014) comYH 971713..972669 (+) 957 WP_011681684.1 class I SAM-dependent methyltransferase Machinery gene
  BBD27_RS05200 (BBD27_1015) - 972725..973918 (+) 1194 WP_014608740.1 acetate kinase -
  BBD27_RS05205 - 974167..974365 (+) 199 Protein_966 helix-turn-helix transcriptional regulator -
  BBD27_RS11455 (BBD27_1017) - 974377..974652 (+) 276 WP_011681681.1 hypothetical protein -
  BBD27_RS11460 (BBD27_1018) - 974696..975034 (+) 339 WP_224103197.1 CPBP family intramembrane glutamic endopeptidase -
  BBD27_RS05220 (BBD27_1019) - 975108..975551 (+) 444 WP_011681680.1 hypothetical protein -
  BBD27_RS05225 (BBD27_1020) - 975648..976310 (+) 663 WP_011681679.1 CPBP family intramembrane glutamic endopeptidase -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 16375.85 Da        Isoelectric Point: 8.4500

>NTDB_id=187845 BBD27_RS05185 WP_011681686.1 970936..971373(+) (comYF) [Streptococcus thermophilus strain ND07]
MYLSSKLRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVRQQENQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSASDFRKTNADGRGYQPMLFDVKSSKVSQKNQIVTIQVTLKNELQRSFVYAFEKTG

Nucleotide


Download         Length: 438 bp        

>NTDB_id=187845 BBD27_RS05185 WP_011681686.1 970936..971373(+) (comYF) [Streptococcus thermophilus strain ND07]
ATGTATCTAAGCAGTAAGCTGCGTGCCTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTCATTGCTGGCTCAGTTCA
AGTTTATCAAGGGTTAACCACTGTACTTGTGAGTAATGTTAAGCAGGTAAGACAACAAGAAAACCAAGATTGGCTTCTAT
TTGTTCAACAAATGGAGGCAGAATTAGAAGGATGTCAGTTGGTCAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTGGCTTTTGGTCTTTCAAGTGCCTCTGATTTTCGAAAAACAAATGCTGATGGTCGAGGTTATCAACCAAT
GCTTTTCGATGTCAAATCTAGTAAGGTAAGTCAAAAGAACCAGATAGTTACCATCCAAGTGACTCTTAAAAATGAACTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

58.156

97.241

0.566

  comYF Streptococcus mutans UA159

57.447

97.241

0.559

  comGF/cglF Streptococcus pneumoniae D39

49.286

96.552

0.476

  comGF/cglF Streptococcus pneumoniae Rx1

49.286

96.552

0.476

  comGF/cglF Streptococcus pneumoniae R6

49.286

96.552

0.476

  comGF/cglF Streptococcus pneumoniae TIGR4

49.286

96.552

0.476

  comGF/cglF Streptococcus mitis NCTC 12261

47.518

97.241

0.462

  comGF/cglF Streptococcus mitis SK321

46.809

97.241

0.455

  comGF Lactococcus lactis subsp. cremoris KW2

45.324

95.862

0.434


Multiple sequence alignment