Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AOT16_RS16410 Genome accession   NZ_CP016295
Coordinates   3390826..3391626 (-) Length   266 a.a.
NCBI ID   WP_002027454.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain CMC-CR-MDR-Ab4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3385826..3396626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT16_RS16390 (AOT16_16120) - 3386891..3387589 (-) 699 WP_000058788.1 YfgM family protein -
  AOT16_RS16395 (AOT16_16125) hisS 3387618..3388910 (-) 1293 WP_000095264.1 histidine--tRNA ligase -
  AOT16_RS16400 (AOT16_16130) ispG 3388907..3390022 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AOT16_RS16405 (AOT16_16135) - 3390044..3390835 (-) 792 WP_000405164.1 helix-turn-helix domain-containing protein -
  AOT16_RS16410 (AOT16_16140) pilF 3390826..3391626 (-) 801 WP_002027454.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AOT16_RS16415 (AOT16_16145) rlmN 3391642..3392874 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  AOT16_RS16420 (AOT16_16150) ndk 3392997..3393428 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  AOT16_RS16425 (AOT16_16155) iscX 3393542..3393742 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  AOT16_RS16430 (AOT16_16160) - 3393842..3394393 (-) 552 WP_000669567.1 phosphatase PAP2 family protein -
  AOT16_RS16435 (AOT16_16165) - 3394418..3395716 (-) 1299 WP_000348500.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30124.47 Da        Isoelectric Point: 9.2307

>NTDB_id=186605 AOT16_RS16410 WP_002027454.1 3390826..3391626(-) (pilF) [Acinetobacter baumannii strain CMC-CR-MDR-Ab4]
MSTPKLKIMLCMGVAVALLTSGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=186605 AOT16_RS16410 WP_002027454.1 3390826..3391626(-) (pilF) [Acinetobacter baumannii strain CMC-CR-MDR-Ab4]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGACAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCATCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCCTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment