Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BAU07_RS00530 Genome accession   NZ_CP016172
Coordinates   113571..115007 (+) Length   478 a.a.
NCBI ID   WP_066652608.1    Uniprot ID   A0A193G969
Organism   Bordetella flabilis strain AU10664     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 108571..120007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BAU07_RS00510 (BAU07_00510) - 108878..109753 (+) 876 WP_066652600.1 carbohydrate ABC transporter permease -
  BAU07_RS00515 (BAU07_00515) - 109750..110697 (+) 948 WP_232338214.1 carbohydrate ABC transporter permease -
  BAU07_RS00520 (BAU07_00520) leuA 110728..112443 (-) 1716 WP_066652601.1 2-isopropylmalate synthase -
  BAU07_RS00525 (BAU07_00525) - 112898..113572 (-) 675 WP_066652603.1 hypothetical protein -
  BAU07_RS00530 (BAU07_00530) rpoN/rpoN1 113571..115007 (+) 1437 WP_066652608.1 RNA polymerase factor sigma-54 Machinery gene
  BAU07_RS26615 - 115101..115373 (+) 273 WP_084025042.1 (2Fe-2S)-binding protein -
  BAU07_RS00535 (BAU07_00535) - 115319..116233 (-) 915 WP_066652610.1 LysR substrate-binding domain-containing protein -
  BAU07_RS00540 (BAU07_00540) - 116357..117274 (+) 918 WP_066652612.1 DMT family transporter -
  BAU07_RS00545 (BAU07_00545) bfr 117500..117976 (+) 477 WP_066652613.1 bacterioferritin -
  BAU07_RS00550 (BAU07_00550) - 118035..118724 (-) 690 WP_066652618.1 hypothetical protein -

Sequence

Protein


Download         Length: 478 a.a.        Molecular weight: 52656.09 Da        Isoelectric Point: 5.3930

>NTDB_id=185972 BAU07_RS00530 WP_066652608.1 113571..115007(+) (rpoN/rpoN1) [Bordetella flabilis strain AU10664]
MQNIVPMTRHALELRPGQHLALTPQLQQSIRMLQLSTLDLETEISQALSDNPLLERDEDPRGDDVDPEREGSAHDDEPAP
ERETGVDEMPGAKGVYSDDDSEMPEQARAETLREHLLAQLSLTRAGQRDAALTALLIDELDENGYLASPLDEILSWLPAE
IEADIDELRAALRLLQSFDPPGVGAADMAECLDLQLRRPDVAVLPEARDPAVLACARRICAEHLPLLAGNAARLREALQC
DDAMLRGARALILKLDPRPGRKWTVPAADYAIPDVIVRKGRKGWHVVLNSAAMPRLRINGVYARMLGNQREASHAALHAQ
LQQARWMIRNVEQRFDTILRVAQAIVNHQAAFFSQGPSAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTMELKDF
FGAGVATDSGDAASATAVQAFIRRLVADENRAKPLSDNQIMLKLAEQGIVIARRTVAKYREAMRIAPASVRRAQAAAR

Nucleotide


Download         Length: 1437 bp        

>NTDB_id=185972 BAU07_RS00530 WP_066652608.1 113571..115007(+) (rpoN/rpoN1) [Bordetella flabilis strain AU10664]
ATGCAAAATATTGTACCTATGACTCGCCACGCCTTAGAACTCCGGCCAGGACAACATCTTGCCCTGACCCCGCAATTGCA
GCAGTCCATCCGGATGCTGCAGTTGTCGACGCTCGACCTCGAGACGGAGATCTCACAGGCCTTGTCCGATAATCCGTTGC
TCGAACGCGACGAAGACCCGCGCGGCGACGACGTCGATCCGGAACGCGAGGGCTCAGCCCATGACGACGAACCGGCACCA
GAACGGGAAACCGGAGTCGACGAAATGCCGGGCGCCAAGGGCGTGTACTCGGACGACGACAGCGAGATGCCCGAACAGGC
GCGCGCCGAAACCTTGCGCGAGCACTTGCTGGCCCAACTGTCGCTGACCCGCGCGGGTCAGCGCGATGCGGCGCTCACCG
CGCTGCTGATCGACGAACTGGACGAGAACGGCTATCTGGCTTCGCCGTTGGACGAAATCCTGTCCTGGCTGCCGGCCGAG
ATCGAGGCCGACATCGACGAGCTGCGGGCCGCGCTGCGCTTGCTGCAATCCTTCGACCCGCCGGGCGTCGGGGCGGCCGA
CATGGCGGAATGCCTGGACCTGCAACTGCGCCGTCCGGACGTTGCCGTGCTTCCCGAGGCCCGCGATCCGGCCGTGCTCG
CCTGTGCGCGGAGGATCTGCGCCGAACACCTGCCGCTGCTGGCCGGCAATGCCGCGCGCCTGCGCGAAGCGCTGCAGTGC
GACGACGCCATGCTGCGCGGCGCGCGCGCCCTGATACTGAAACTGGATCCGCGGCCCGGCCGCAAATGGACGGTCCCGGC
GGCGGACTACGCCATTCCCGATGTCATCGTGCGCAAGGGCCGCAAAGGCTGGCATGTGGTCCTGAACAGCGCGGCGATGC
CGCGCCTGCGCATCAACGGGGTCTATGCACGCATGCTGGGCAACCAGCGCGAGGCCAGCCACGCCGCCCTGCACGCCCAA
TTGCAGCAGGCGCGGTGGATGATCCGCAACGTGGAACAGCGTTTCGACACCATCCTGCGCGTGGCCCAGGCCATCGTGAA
TCACCAGGCGGCGTTCTTCAGCCAGGGGCCGTCGGCCATGCGGCCGCTTATCCTCAAGGACATCGCCGGCGAGCTTGGCC
TGCACGAATCCACCATCTCGCGGGCGACCACCCAGAAGTACATGCTGACGCCGTTCGGCACGATGGAGCTGAAGGATTTC
TTCGGCGCGGGCGTCGCCACGGACAGCGGCGACGCCGCCTCGGCCACGGCCGTGCAGGCCTTCATCCGCCGCCTGGTGGC
GGACGAGAACCGCGCCAAGCCGCTTTCCGACAATCAGATCATGCTGAAACTGGCCGAACAAGGTATTGTCATCGCGCGCC
GTACGGTGGCCAAATACCGCGAAGCCATGCGCATCGCGCCGGCATCGGTGCGCCGGGCCCAGGCCGCAGCACGCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-472)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(106-302)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A193G969

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

47.826

100

0.506

Multiple sequence alignment