Detailed information NTG0185476

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: A9P85_RS04845
• Genome accession: NZ_CP016029
• Coordinates: 1078239..1079303 (+)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila strain Pontiac
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1073239..1084303

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A9P85_RS04835 (A9P85_04835)	-	1074279..1076663 (-)	2385	WP_027220940.1	penicillin-binding protein 1A	-
A9P85_RS04840 (A9P85_04840)	ravI	1076815..1077822 (-)	1008	WP_011946020.1	Dot/Icm T4SS effector RavI	-
A9P85_RS04845 (A9P85_04845)	pilM	1078239..1079303 (+)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
A9P85_RS04850 (A9P85_04850)	pilN	1079307..1079855 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
A9P85_RS04855 (A9P85_04855)	pilO	1079869..1080468 (+)	600	WP_011213355.1	type 4a pilus biogenesis protein PilO	Machinery gene
A9P85_RS04860 (A9P85_04860)	pilP	1080465..1081049 (+)	585	WP_011213356.1	pilus assembly protein PilP	Machinery gene
A9P85_RS04865 (A9P85_04865)	pilQ	1081053..1083149 (+)	2097	WP_027220941.1	type IV pilus secretin PilQ	Machinery gene
A9P85_RS04870 (A9P85_04870)	aroK	1083595..1084122 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=185476 A9P85_RS04845 WP_011215094.1 1078239..1079303(+) (pilM) [Legionella pneumophila strain Pontiac]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=185476 A9P85_RS04845 WP_011215094.1 1078239..1079303(+) (pilM) [Legionella pneumophila strain Pontiac]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGCATCAAAAAATTGGTAGAAAGATTGAAGTTTACATCTAAAAATGTAGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAACTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAGCACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCACCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407