Detailed information NTG0185124

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: AM483_RS09260
• Genome accession: NZ_CP020116
• Coordinates: 1821003..1822013 (+)
• Length: 336 a.a.
• NCBI ID: WP_000568520.1
• Uniprot ID: -
• Organism: Escherichia coli strain AR_0104
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1816003..1827013

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AM483_RS09230 (AM483_09415)	nudB	1817727..1818179 (+)	453	WP_077249130.1	dihydroneopterin triphosphate diphosphatase	-
AM483_RS09235 (AM483_09420)	yebC	1818208..1818948 (+)	741	WP_000907234.1	YebC/PmpR family DNA-binding transcriptional regulator	-
AM483_RS09240 (AM483_09425)	ruvC	1818983..1819504 (+)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
AM483_RS09245 (AM483_09430)	yebB	1819506..1820108 (-)	603	WP_001024911.1	YebB family permuted papain-like enzyme	-
AM483_RS09250 (AM483_09435)	yobI	1820179..1820244 (+)	66	WP_072093883.1	stress response small protein YobI	-
AM483_RS09255 (AM483_09440)	ruvA	1820383..1820994 (+)	612	WP_000580323.1	Holliday junction branch migration protein RuvA	-
AM483_RS09260 (AM483_09445)	ruvB	1821003..1822013 (+)	1011	WP_000568520.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
AM483_RS27700	-	1822123..1823471 (+)	1349	WP_099156422.1	IS3-like element IS1397 family transposase	-
AM483_RS09280 (AM483_09465)	znuB	1823665..1824450 (-)	786	WP_000571478.1	zinc ABC transporter permease subunit ZnuB	-
AM483_RS09285 (AM483_09470)	znuC	1824447..1825202 (-)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
AM483_RS09290 (AM483_09475)	znuA	1825281..1826213 (+)	933	WP_001347089.1	zinc ABC transporter substrate-binding protein ZnuA	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37163.73 Da
• Isoelectric Point: 4.7818

>NTDB_id=185124 AM483_RS09260 WP_000568520.1 1821003..1822013(+) (ruvB) [Escherichia coli strain AR_0104]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMS

Nucleotide

• Length: 1011 bp

>NTDB_id=185124 AM483_RS09260 WP_000568520.1 1821003..1822013(+) (ruvB) [Escherichia coli strain AR_0104]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGCGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGTCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494