Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   EC725_RS12140 Genome accession   NZ_CP020092
Coordinates   2297158..2298168 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain 13E0725     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2292158..2303168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EC725_RS12110 (EC725_12110) nudB 2293882..2294334 (+) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -
  EC725_RS12115 (EC725_12115) yebC 2294363..2295103 (+) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  EC725_RS12120 (EC725_12120) ruvC 2295138..2295659 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  EC725_RS12125 (EC725_12125) yebB 2295661..2296263 (-) 603 WP_001024929.1 YebB family permuted papain-like enzyme -
  EC725_RS12130 (EC725_12130) yobI 2296334..2296399 (+) 66 WP_001386853.1 stress response small protein YobI -
  EC725_RS12135 (EC725_12135) ruvA 2296538..2297149 (+) 612 WP_033816181.1 Holliday junction branch migration protein RuvA -
  EC725_RS12140 (EC725_12140) ruvB 2297158..2298168 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  EC725_RS12145 (EC725_12145) znuB 2298315..2299100 (-) 786 WP_000571465.1 zinc ABC transporter permease subunit ZnuB -
  EC725_RS12150 (EC725_12150) znuC 2299097..2299852 (-) 756 WP_032314091.1 zinc ABC transporter ATP-binding protein ZnuC -
  EC725_RS12155 (EC725_12155) znuA 2299931..2300863 (+) 933 WP_001300644.1 zinc ABC transporter substrate-binding protein ZnuA -
  EC725_RS12160 (EC725_12160) mepM 2300879..2302201 (+) 1323 WP_032314092.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=184745 EC725_RS12140 WP_000568519.1 2297158..2298168(+) (ruvB) [Escherichia coli strain 13E0725]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=184745 EC725_RS12140 WP_000568519.1 2297158..2298168(+) (ruvB) [Escherichia coli strain 13E0725]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTGCGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGACTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCACGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494