Detailed information NTG0184547

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: B5D85_RS00330
• Genome accession: NZ_CP020082
• Coordinates: 54612..55610 (+)
• Length: 332 a.a.
• NCBI ID: WP_002986679.1
• Uniprot ID: A2RC05
• Organism: Streptococcus pyogenes strain STAB120304
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 49612..60610

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B5D85_RS00315 (B5D85_00315)	-	50343..52040 (+)	1698	WP_020904816.1	AIPR family protein	-
B5D85_RS00320 (B5D85_00320)	purB	52058..53350 (+)	1293	WP_020904817.1	adenylosuccinate lyase	-
B5D85_RS00325 (B5D85_00325)	comR	53481..54392 (+)	912	WP_020904818.1	helix-turn-helix domain-containing protein	Regulator
B5D85_RS10025	comS	54483..54581 (+)	99	WP_020833188.1	quorum-sensing system DWW-type pheromone	Regulator
B5D85_RS00330 (B5D85_00330)	ruvB	54612..55610 (+)	999	WP_002986679.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
B5D85_RS00335 (B5D85_00335)	-	55748..56185 (+)	438	WP_020904819.1	low molecular weight protein-tyrosine-phosphatase	-
B5D85_RS00340 (B5D85_00340)	-	56209..56610 (+)	402	WP_002986671.1	MORN repeat-containing protein	-
B5D85_RS00345 (B5D85_00345)	-	56607..58382 (+)	1776	WP_076639715.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37565.11 Da
• Isoelectric Point: 5.3005

>NTDB_id=184547 B5D85_RS00330 WP_002986679.1 54612..55610(+) (ruvB) [Streptococcus pyogenes strain STAB120304]
MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=184547 B5D85_RS00330 WP_002986679.1 54612..55610(+) (ruvB) [Streptococcus pyogenes strain STAB120304]
ATGGCTAGAATTTTAGATAATAATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACACGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCTATTTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGACTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCAGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCAGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTCGAGGAAGTACTATATAGTGCTATGGAAGACTTCTATATTGATA
TTATGATTGGCGCTGGAGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGATTATGCTCAAATTATTGGTGATGGCATCATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCGATTACATTGATCAGAAAATTTTACG
AACGATGATTGAGATGTATCAGGGAGGACCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACGCAAAAG
GCTTATCGTCATCTTGGCTATCCCTATCAAAACACTTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: A2RC05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae D39	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae TIGR4	84.545	99.398	0.84
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.824	97.289	0.572
ruvB	Helicobacter pylori 26695	48.936	99.096	0.485
ruvB	Synechocystis sp. PCC 6803	50.16	94.277	0.473