Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Vc3S01_RS02190 Genome accession   NZ_CP020076
Coordinates   453374..455098 (+) Length   574 a.a.
NCBI ID   WP_077200166.1    Uniprot ID   -
Organism   Vibrio campbellii strain 20130629003S01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 448374..460098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Vc3S01_RS02165 (Vc3S01_0381) gspM 448780..449430 (+) 651 WP_038891137.1 type II secretion system protein GspM -
  Vc3S01_RS02170 (Vc3S01_0382) - 449423..449752 (+) 330 WP_005533781.1 hypothetical protein -
  Vc3S01_RS02175 (Vc3S01_0383) mshL 449773..451407 (+) 1635 WP_045382715.1 pilus (MSHA type) biogenesis protein MshL -
  Vc3S01_RS02180 (Vc3S01_0384) - 451450..452295 (+) 846 WP_077200164.1 AAA family ATPase -
  Vc3S01_RS02185 (Vc3S01_0385) - 452280..453377 (+) 1098 WP_077200165.1 tetratricopeptide repeat protein -
  Vc3S01_RS02190 (Vc3S01_0386) pilB 453374..455098 (+) 1725 WP_077200166.1 GspE/PulE family protein Machinery gene
  Vc3S01_RS02195 (Vc3S01_0387) - 455107..456330 (+) 1224 WP_077200167.1 type II secretion system F family protein -
  Vc3S01_RS02200 (Vc3S01_0388) - 456335..456796 (+) 462 WP_047482420.1 hypothetical protein -
  Vc3S01_RS02205 (Vc3S01_0389) - 457100..457567 (+) 468 WP_077200168.1 type II secretion system protein -
  Vc3S01_RS02210 (Vc3S01_0390) - 457638..457868 (+) 231 WP_005432800.1 MSHA biogenesis protein MshA -
  Vc3S01_RS02215 (Vc3S01_0391) - 457988..458473 (+) 486 WP_077200169.1 type II secretion system protein -
  Vc3S01_RS02220 (Vc3S01_0392) - 458470..459039 (+) 570 WP_077200170.1 type II secretion system protein -
  Vc3S01_RS02225 (Vc3S01_0393) - 459040..459753 (+) 714 WP_045454928.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63808.09 Da        Isoelectric Point: 4.9676

>NTDB_id=184476 Vc3S01_RS02190 WP_077200166.1 453374..455098(+) (pilB) [Vibrio campbellii strain 20130629003S01]
MKIQLRKRLGDLLVEEGIVSEDQVQQALSAQHSTGQKLGDALIDLGFISEKQMLEFLSQQLGLPLIDLSRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYDLEFIIAPERQLVESFDRYYRRTKEIASFAEQLQAE
HQDVEGFDYGIDEGDSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETLLNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRKLEDSGLPPELLARLRRQLARPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTTDVLDESRQQWLAGRFPNQI
GTTFHKGAGCQNCNLTGYRGRVGVFEMLELENEMMDKLRANDAVGFAQVARRSENYKPLLASAMELALQGTVSLDEVMAL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=184476 Vc3S01_RS02190 WP_077200166.1 453374..455098(+) (pilB) [Vibrio campbellii strain 20130629003S01]
ATGAAAATTCAGTTGAGAAAACGCTTAGGCGACCTATTGGTTGAAGAGGGTATTGTCTCTGAAGATCAGGTCCAGCAAGC
CCTGAGTGCTCAGCATAGCACCGGACAAAAGTTAGGTGACGCTCTTATCGATCTTGGCTTTATCAGTGAAAAACAGATGC
TGGAGTTTTTGTCTCAGCAACTTGGCTTACCATTGATCGATTTAAGCCGTGCGCCTGTTGATGCTGATGCTGTACAGATC
TTGCCAGAGGTGCATGCTCGCCGCTTACGTGCCATGGTTGTGGCTCGTAATGGTGACACGCTGCGTGTTGCCATGAGTGA
CCCTGCTGATCTATTTACCCAAGAGTCATTGATGAACTTGCTCGGTGAGTACGATTTAGAATTTATCATTGCACCTGAAC
GCCAGTTAGTAGAAAGCTTTGACCGTTACTATCGCCGAACCAAAGAAATCGCCTCGTTTGCTGAACAGCTGCAAGCTGAG
CACCAAGATGTTGAAGGCTTTGATTACGGCATTGATGAAGGCGACAGCGAAGAAGTTACCGTTGTAAAACTGGTTAACTC
GATGTTTGAAGATGCTGTCCAAGTTGGCGCGTCGGATATTCATATTGAGCCAGACGACAAGGTGCTGCGTTTACGTCAAC
GTGTCGATGGTGTACTCCATGAGACACTTCTAAACGAGGTGAATATTGCCTCTGCGCTAGTATTACGTCTGAAGCTGATG
GCGCATCTCGATATCTCTGAAAAGCGCTTACCTCAAGATGGTCGATTCAATATCAAAGTTCGTGGTCAATCGATCGATAT
TCGTATGTCTACTCTGCCAACGCAATACGGTGAATCAGTAGTAATGCGTTTGCTAAACCAATCTTCTGGTTTGCGTAAGC
TTGAGGATTCTGGCTTACCACCTGAGTTGTTAGCACGTCTTCGTCGTCAACTTGCTCGCCCTCACGGCATGATCCTAGTT
ACGGGGCCGACAGGTTCAGGTAAAACCACCACGCTTTATGGTGCGTTGAGTGAATTAAACGAGCCGGGCAAAAAGATCAT
CACCGCAGAAGATCCGGTGGAATACCGCTTACCGCGTATTACTCAGGTGCAGATCAACAGTAAAATTGATCTGACTTTTT
CGCGAGTGCTGCGTACCTTCCTACGTCAAGACCCAGATATCATCCTTATCGGTGAGATGCGTGACCAAGAAACGGTTGAG
ATTGGCCTCCGTGCTGCACTAACTGGTCACTTAGTACTAAGTACCTTGCACACTAACGATGCGGTAGATAGTGCGCTGCG
AATGATTGATATGGGTGCTCCGGGTTATTTGGTCGCGAGTGCTGTTCGTGCGGTTGTGGCTCAACGTCTTGTTCGTCGTG
TCTGCCCTGACTGTAAAACAACGGATGTGCTCGATGAGTCTCGTCAGCAGTGGCTTGCGGGGCGCTTCCCAAATCAAATC
GGCACTACTTTCCACAAAGGTGCAGGTTGTCAAAACTGTAACCTGACCGGTTATCGTGGCCGAGTTGGTGTGTTTGAAAT
GCTCGAGCTAGAAAATGAGATGATGGACAAGCTGCGTGCTAATGATGCTGTAGGCTTTGCTCAAGTGGCGCGCCGCTCAG
AAAACTATAAGCCTCTGTTGGCTTCTGCTATGGAGCTTGCACTGCAAGGTACCGTGAGTTTGGATGAAGTCATGGCCCTG
GGTGAGGGCGATGCTTCTGGTAAAACCGACCCAATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.681

97.735

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

35.42

100

0.375
  pilB Acinetobacter baumannii D1279779

36.907

99.129

0.366
  pilF Thermus thermophilus HB27

37.5

97.561

0.366
  pilB Legionella pneumophila strain ERS1305867

36.926

98.606

0.364

Multiple sequence alignment