Detailed information NTG0184039

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: A9E80_RS04575
• Genome accession: NZ_CP015935
• Coordinates: 1003909..1004973 (+)
• Length: 354 a.a.
• NCBI ID: WP_010946662.1
• Uniprot ID: Q5ZX06
• Organism: Legionella pneumophila strain C4_S
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 998909..1009973

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A9E80_RS04565 (A9E80_04565)	-	999946..1002330 (-)	2385	WP_010946660.1	penicillin-binding protein 1A	-
A9E80_RS04570 (A9E80_04570)	ravI	1002482..1003492 (-)	1011	WP_010946661.1	Dot/Icm T4SS effector RavI	-
A9E80_RS04575 (A9E80_04575)	pilM	1003909..1004973 (+)	1065	WP_010946662.1	type IV pilus assembly protein PilM	Machinery gene
A9E80_RS04580 (A9E80_04580)	pilN	1004977..1005525 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
A9E80_RS04585 (A9E80_04585)	pilO	1005539..1006138 (+)	600	WP_014841277.1	type 4a pilus biogenesis protein PilO	Machinery gene
A9E80_RS04590 (A9E80_04590)	pilP	1006135..1006722 (+)	588	WP_010946665.1	pilus assembly protein PilP	Machinery gene
A9E80_RS04595 (A9E80_04595)	pilQ	1006726..1008822 (+)	2097	WP_010946666.1	type IV pilus secretin PilQ	Machinery gene
A9E80_RS04600 (A9E80_04600)	aroK	1009268..1009795 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38732.71 Da
• Isoelectric Point: 5.0367

>NTDB_id=184039 A9E80_RS04575 WP_010946662.1 1003909..1004973(+) (pilM) [Legionella pneumophila strain C4_S]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNIIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQSGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=184039 A9E80_RS04575 WP_010946662.1 1003909..1004973(+) (pilM) [Legionella pneumophila strain C4_S]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATCCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGCGAAGAAATACCTGCCTCTGCCTTAGAAGGAAATATAA
TTAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATCGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAATCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5ZX06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.435	100	0.994
comM	Acinetobacter nosocomialis M2	42.817	100	0.429
pilM	Acinetobacter baumannii D1279779	42.535	100	0.427
comM	Acinetobacter baylyi ADP1	41.457	100	0.418