Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PS273GM_RS10560 Genome accession   NZ_CP015641
Coordinates   2315941..2318052 (+) Length   703 a.a.
NCBI ID   WP_064481359.1    Uniprot ID   A0A172WQ36
Organism   Stutzerimonas stutzeri strain 273     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2310941..2323052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PS273GM_RS10540 (PS273GM_10540) comM 2313109..2314173 (+) 1065 WP_045430802.1 pilus assembly protein PilM Machinery gene
  PS273GM_RS10545 (PS273GM_10545) pilN 2314173..2314745 (+) 573 WP_045430800.1 type 4a pilus biogenesis protein PilN -
  PS273GM_RS10550 (PS273GM_10550) pilO 2314742..2315365 (+) 624 WP_045430797.1 type 4a pilus biogenesis protein PilO -
  PS273GM_RS10555 (PS273GM_10555) pilP 2315362..2315889 (+) 528 WP_045430794.1 type 4a pilus biogenesis lipoprotein PilP -
  PS273GM_RS10560 (PS273GM_10560) pilQ 2315941..2318052 (+) 2112 WP_064481359.1 type IV pilus secretin PilQ Machinery gene
  PS273GM_RS10565 (PS273GM_10565) aroK 2318085..2318603 (+) 519 WP_045430792.1 shikimate kinase AroK -
  PS273GM_RS10570 (PS273GM_10570) aroB 2318627..2319718 (+) 1092 WP_045430790.1 3-dehydroquinate synthase -
  PS273GM_RS10575 (PS273GM_10575) - 2319739..2321232 (+) 1494 WP_045430788.1 AAA family ATPase -

Sequence

Protein


Download         Length: 703 a.a.        Molecular weight: 75455.40 Da        Isoelectric Point: 4.8002

>NTDB_id=181651 PS273GM_RS10560 WP_064481359.1 2315941..2318052(+) (pilQ) [Stutzerimonas stutzeri strain 273]
MNSTLTRLGLSLLAAFISPALLAANLNSLDVASLPGDRVELKLAFDEPVPTPRGYTLDQPARIALDLPGVSSKLGSKNHE
LGVGNARSVTVVEAQGRTRLIVNLTSLVPYSTRVDGNNLFVVLGEAASAPAAASAAPAVSTKPVTPNASLAIGKSISNID
FRRGNDGAGNVVITLSDPSISPSIEEQGGKIRVSFAKTQLPESLRVRLDVQDFATPVKFVDSSSKADGASIAIEPSGRYD
YLAYQTDDKLTISVKPLSEADAEQRKAEHFTYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPW
DQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESKRQIAELAPLRREVVQVNYAKAADIAKLFQSVTAVDGQAD
ERGSIAVDDRTNNIIAYQTQERLDELRRIVTQLDIPVRQVMIEARIVEANVGYDKALGVRWGGSAVNDQWSAYGKDGSRS
IDDDGVLRLPGSDTVGNFTLDEGVAPVPFVDMGVLGSTSGVGIGFISDHLLLDLQLSAMEKTGNGEVVSQPKVMTADKET
AKILKGTEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFRNAVNGVPPIDKNEVNAKVLVNDG
ETVVIGGVFSNSQSKSVDKVPFLGDVPYLGRLFRRDVVTDAKSELLVFLTPRILNHQAIAVNR

Nucleotide


Download         Length: 2112 bp        

>NTDB_id=181651 PS273GM_RS10560 WP_064481359.1 2315941..2318052(+) (pilQ) [Stutzerimonas stutzeri strain 273]
ATGAATAGCACTCTCACTCGTCTCGGTTTGTCGTTGCTGGCAGCGTTCATTTCGCCTGCATTGCTGGCAGCAAATTTGAA
TTCACTTGATGTCGCATCGTTGCCCGGCGACCGCGTTGAGCTGAAGCTGGCTTTCGACGAACCGGTTCCCACTCCTCGTG
GCTATACGCTCGACCAGCCAGCCCGCATCGCTCTTGATCTGCCAGGCGTATCCAGCAAGCTGGGGTCGAAAAATCATGAG
CTGGGTGTCGGTAATGCGCGAAGCGTGACCGTGGTGGAGGCGCAGGGGCGTACCCGACTCATCGTGAATCTCACTTCTCT
GGTTCCGTACTCGACCCGTGTTGACGGCAACAACCTGTTCGTCGTGCTTGGCGAAGCCGCTAGCGCCCCAGCTGCTGCTT
CAGCTGCTCCGGCGGTCTCGACGAAACCGGTCACACCGAACGCTTCGCTGGCTATTGGAAAATCCATCAGCAATATCGAT
TTTCGCCGTGGAAATGACGGGGCCGGTAATGTGGTCATCACCTTGTCTGACCCTTCGATCAGCCCAAGTATTGAAGAGCA
AGGCGGTAAAATTCGAGTTTCCTTCGCCAAGACGCAATTGCCGGAATCGCTGCGTGTTCGACTGGATGTCCAAGACTTTG
CTACGCCAGTTAAATTTGTCGATTCAAGTAGCAAGGCAGATGGCGCCAGTATTGCTATCGAGCCCAGCGGTCGATACGAC
TACCTGGCGTACCAGACTGACGACAAGCTGACCATTAGTGTCAAGCCGCTCAGCGAGGCAGATGCCGAGCAACGCAAGGC
CGAACATTTCACCTACTCCGGTGAGAAGTTGTCGCTGAATTTCCAGGACATTGACGTTCGCTCTGTTCTGCAGCTAATTG
CGGATTTTACTGATCTGAATCTGGTGGCTAGCGATACCGTACAGGGCAATATCACTCTACGTTTGCAGAACGTACCCTGG
GATCAGGCGCTGGATCTCGTGCTGAAAACCAAAGGCCTCGACAAACGCCAGGTGGGGAATGTTCTGCTGGTGGCTCCGGC
AGACGAGATCGCCGCGAGGGAGCGTCAAGAGTTGGAGTCCAAGCGGCAAATTGCCGAGCTGGCGCCCCTCCGTCGTGAAG
TCGTGCAGGTGAATTACGCTAAGGCAGCGGATATTGCGAAGCTCTTTCAGTCGGTGACTGCGGTCGATGGGCAGGCTGAC
GAGCGCGGCTCCATCGCGGTTGATGACCGCACAAACAACATAATCGCCTACCAAACCCAAGAACGTTTGGATGAACTACG
CCGTATCGTAACTCAGCTGGATATCCCAGTTCGCCAGGTCATGATTGAGGCGCGGATTGTTGAGGCAAACGTTGGCTATG
ATAAAGCGCTTGGGGTGCGGTGGGGCGGTAGCGCTGTGAACGATCAGTGGAGTGCCTATGGCAAGGATGGCAGCAGATCT
ATCGACGATGATGGGGTTTTGCGGCTACCCGGTTCAGATACCGTTGGTAACTTCACGCTCGATGAAGGCGTGGCCCCAGT
CCCCTTTGTTGACATGGGAGTACTAGGGAGCACTTCCGGCGTGGGCATTGGCTTTATTTCGGATCACCTCTTATTGGATC
TTCAACTCTCTGCGATGGAGAAGACCGGAAATGGAGAAGTCGTATCCCAGCCCAAGGTTATGACAGCGGACAAAGAAACA
GCGAAGATTTTGAAGGGCACAGAAGTGCCCTATCAGGAGGCCAGCTCAAGCGGTGCGACTAGCACCTCTTTTAAAGAGGC
GGCCTTATCCTTGGAGGTAACCCCGCAGATTACGCCGGATAATCGCATCATCATGGAGGTCAAGGTGACGAAGGATGCGC
CAGATTTCCGCAACGCGGTTAACGGTGTACCTCCAATCGATAAAAACGAAGTCAATGCCAAGGTTCTGGTAAACGATGGA
GAAACTGTCGTGATCGGTGGTGTGTTCTCCAACAGCCAGTCGAAAAGCGTCGACAAGGTTCCGTTTCTGGGCGATGTGCC
TTATCTTGGCCGCTTGTTTCGTCGCGATGTCGTGACGGATGCCAAGTCTGAATTGCTGGTGTTCCTTACACCTAGAATCC
TGAATCATCAGGCCATCGCCGTGAATCGTTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(373-439)

Bacterial type II and III secretion system protein (Secretin)

(538-694)

Secretin and TonB N terminus short domain (STN)

(300-346)

AMIN domain (AMIN)

(38-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172WQ36

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.37

100

0.755
  pilQ Acinetobacter baumannii strain A118

40.136

100

0.42
  pilQ Acinetobacter baumannii D1279779

40.136

100

0.42
  comQ Acinetobacter baylyi ADP1

42.341

98.435

0.417

Multiple sequence alignment