Detailed information NTG0181524

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AsACE_RS07610
• Genome accession: NZ_CP015615
• Coordinates: 1595816..1596994 (-)
• Length: 392 a.a.
• NCBI ID: WP_005222289.1
• Uniprot ID: N9N6B8
• Organism: Acinetobacter schindleri strain ACE
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1590816..1601994

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AsACE_RS07590 (AsACE_CH01508)	-	1591259..1592287 (+)	1029	WP_076753628.1	AraC family transcriptional regulator	-
AsACE_RS07595 (AsACE_CH01509)	gcvH	1592360..1592734 (+)	375	WP_004809515.1	glycine cleavage system protein GcvH	-
AsACE_RS07600 (AsACE_CH01510)	-	1592964..1594142 (+)	1179	WP_005222285.1	MFS transporter	-
AsACE_RS07605 (AsACE_CH01511)	pilU	1594200..1595405 (-)	1206	WP_076753629.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AsACE_RS07610 (AsACE_CH01512)	pilU	1595816..1596994 (-)	1179	WP_005222289.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AsACE_RS07615 (AsACE_CH01513)	-	1597238..1598287 (-)	1050	WP_076753630.1	3-deoxy-7-phosphoheptulonate synthase	-
AsACE_RS07620 (AsACE_CH01514)	-	1598809..1599339 (-)	531	WP_004809525.1	hypothetical protein	-
AsACE_RS07625 (AsACE_CH01515)	-	1599545..1600657 (-)	1113	WP_004895024.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 44519.14 Da
• Isoelectric Point: 6.6647

>NTDB_id=181524 AsACE_RS07610 WP_005222289.1 1595816..1596994(-) (pilU) [Acinetobacter schindleri strain ACE]
MYTPELLDEARKFMHHMLTKVVEYGGSDLFITADFPPSIKQQGLMKPFGQQKLAADKTRLFAYALMNERQIKEFESVMEC
NFGISVPGVSRFRVNVFQQQHNVGMVIRTITTEIPSFQQLGLPNSLQQVIMEKRGLVLIVGATGVGKSTTLAAMIDHRNT
YSEGHIITIEDPVEYVYQHKKSMITQREIGVDCVSWHNALINTMRQAPDVIVIGEIRDTESMEHAIAFAESGHLCLATLH
SNNADQALDRIINFFPEERRNQLLMDLSANMKAVISQRLIRTEDGQGRRAAVEILLNTPLVSDFIRKGDFYELKTIMARS
RELGMQTFDQALFDLYNQGIISYDEALRNADSSNEMRLQIKLKSSRINPALQEEGSKMRILEQSKETTLSRI

Nucleotide

• Length: 1179 bp

>NTDB_id=181524 AsACE_RS07610 WP_005222289.1 1595816..1596994(-) (pilU) [Acinetobacter schindleri strain ACE]
ATGTATACACCCGAATTGCTCGATGAAGCACGTAAATTTATGCACCATATGCTGACCAAGGTAGTTGAATATGGAGGCTC
CGATCTTTTTATTACCGCAGATTTTCCACCAAGTATCAAACAGCAGGGTCTGATGAAGCCTTTTGGTCAGCAAAAACTGG
CAGCGGATAAAACTCGTTTGTTTGCCTATGCTTTAATGAATGAACGACAGATCAAGGAATTTGAATCAGTGATGGAATGC
AATTTCGGTATCAGTGTACCAGGTGTATCACGCTTCCGGGTCAATGTCTTTCAGCAACAGCATAATGTTGGGATGGTAAT
CAGAACCATTACTACGGAGATTCCAAGCTTTCAGCAATTAGGACTACCAAACTCTTTGCAACAAGTGATTATGGAAAAAC
GTGGGCTGGTTCTGATTGTAGGTGCGACAGGTGTTGGTAAATCTACCACACTTGCAGCCATGATTGATCACCGCAATACC
TATTCTGAAGGACATATTATTACCATAGAAGATCCTGTGGAATACGTGTATCAACATAAAAAGTCCATGATTACCCAACG
TGAAATTGGCGTAGATTGTGTTTCCTGGCATAACGCGCTGATCAATACCATGCGTCAGGCACCGGATGTGATCGTGATTG
GTGAAATCCGGGATACCGAAAGTATGGAGCATGCGATTGCCTTTGCTGAATCCGGTCATCTGTGTTTAGCCACCCTGCAT
TCTAATAATGCCGATCAGGCTTTAGACCGCATTATTAATTTCTTCCCGGAAGAACGCCGCAATCAGCTATTAATGGATTT
ATCCGCCAATATGAAAGCTGTGATTTCGCAACGTTTGATCCGTACCGAGGATGGGCAAGGCCGTAGAGCTGCCGTTGAAA
TTTTATTGAATACGCCACTGGTGTCTGATTTTATCCGTAAAGGAGATTTTTACGAGTTGAAAACTATTATGGCGCGTTCG
CGTGAACTCGGCATGCAGACCTTTGATCAGGCATTATTTGATTTGTATAACCAGGGAATTATTTCTTATGACGAAGCCTT
GCGTAATGCAGATTCAAGTAATGAAATGCGTTTGCAGATCAAATTAAAGAGCAGTCGCATCAATCCCGCTCTTCAGGAAG
AAGGAAGTAAAATGAGGATACTGGAACAGTCTAAGGAAACTACACTTTCCAGAATCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (64-282)

3D structure

• AlphaFold DB: N9N6B8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.333	88.776	0.518
pilU	Acinetobacter baylyi ADP1	55.367	90.306	0.5
pilU	Vibrio cholerae strain A1552	49.859	90.561	0.452
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.413	85.204	0.37