Detailed information    

insolico Bioinformatically predicted

Overview


Name   comFC   Type   Machinery gene
Locus tag   BSA145_RS22250 Genome accession   NZ_CP015607
Coordinates   736204..736860 (+) Length   218 a.a.
NCBI ID   WP_231120697.1    Uniprot ID   -
Organism   Bacillus safensis strain U14-5     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 699213..735835 736204..736860 flank 369


Gene organization within MGE regions


Location: 699213..736860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSA145_RS22540 - 699213..699632 (-) 420 WP_231120693.1 hypothetical protein -
  BSA145_RS03660 (BSA145_03610) - 699629..700570 (-) 942 WP_231120694.1 recombinase family protein -
  BSA145_RS03665 (BSA145_03615) - 700583..701230 (-) 648 WP_075621644.1 LexA family protein -
  BSA145_RS03670 (BSA145_03620) - 701427..701681 (+) 255 WP_075621645.1 helix-turn-helix transcriptional regulator -
  BSA145_RS03675 (BSA145_03625) - 701703..701951 (+) 249 WP_075621646.1 hypothetical protein -
  BSA145_RS22235 - 702252..702413 (+) 162 WP_186303771.1 hypothetical protein -
  BSA145_RS03690 (BSA145_03630) - 702766..703416 (+) 651 WP_075621648.1 Rha family transcriptional regulator -
  BSA145_RS03695 (BSA145_03635) - 703746..704090 (+) 345 WP_075621649.1 hypothetical protein -
  BSA145_RS03700 (BSA145_03640) - 704232..704747 (-) 516 WP_075621650.1 hypothetical protein -
  BSA145_RS03705 (BSA145_03645) - 704881..705639 (+) 759 WP_075621651.1 hypothetical protein -
  BSA145_RS03710 (BSA145_03650) - 705843..706244 (+) 402 WP_075621652.1 hypothetical protein -
  BSA145_RS23150 - 706393..706674 (+) 282 WP_335617734.1 hypothetical protein -
  BSA145_RS23155 - 706742..707035 (+) 294 WP_335617735.1 hypothetical protein -
  BSA145_RS03720 (BSA145_03660) - 707028..707387 (+) 360 WP_075621653.1 replicative helicase loader/inhibitor -
  BSA145_RS03725 (BSA145_03665) dnaB 707384..708712 (+) 1329 WP_075621654.1 replicative DNA helicase -
  BSA145_RS03730 (BSA145_03670) - 708709..708924 (+) 216 WP_075621655.1 hypothetical protein -
  BSA145_RS03735 (BSA145_03675) - 709026..709226 (+) 201 WP_196774866.1 XtrA/YqaO family protein -
  BSA145_RS03740 (BSA145_03680) - 709322..709513 (+) 192 WP_075621656.1 hypothetical protein -
  BSA145_RS03745 (BSA145_03685) - 709531..709965 (+) 435 WP_075621657.1 ArpU family phage packaging/lysis transcriptional regulator -
  BSA145_RS03750 (BSA145_03690) - 710101..710301 (+) 201 WP_142394365.1 hypothetical protein -
  BSA145_RS03755 (BSA145_03695) - 710416..710760 (+) 345 WP_075621659.1 structural protein -
  BSA145_RS03760 (BSA145_03700) - 710890..711075 (+) 186 WP_075621660.1 hypothetical protein -
  BSA145_RS03765 (BSA145_03705) - 711081..711368 (+) 288 WP_075621661.1 hypothetical protein -
  BSA145_RS23240 - 711619..711804 (+) 186 WP_418202167.1 hypothetical protein -
  BSA145_RS03775 (BSA145_03710) - 711815..712225 (+) 411 WP_075621662.1 P27 family phage terminase small subunit -
  BSA145_RS03780 (BSA145_03715) - 712225..713979 (+) 1755 WP_081385831.1 terminase large subunit -
  BSA145_RS03785 (BSA145_03720) - 713991..715169 (+) 1179 WP_075621664.1 phage portal protein -
  BSA145_RS03790 (BSA145_03725) - 715162..715749 (+) 588 WP_155762149.1 HK97 family phage prohead protease -
  BSA145_RS03795 (BSA145_03730) - 715749..717026 (+) 1278 WP_075621666.1 phage major capsid protein -
  BSA145_RS03800 (BSA145_03735) - 717004..717270 (+) 267 WP_075621667.1 head-tail connector protein -
  BSA145_RS03805 (BSA145_03740) - 717260..717622 (+) 363 WP_075621668.1 phage head closure protein -
  BSA145_RS23040 - 717591..717716 (+) 126 WP_257787573.1 hypothetical protein -
  BSA145_RS03810 (BSA145_03745) - 717717..718001 (+) 285 WP_075621669.1 hypothetical protein -
  BSA145_RS03815 (BSA145_03750) gp17 718004..718387 (+) 384 WP_075621670.1 tail completion protein gp17 -
  BSA145_RS03820 (BSA145_03755) - 718384..719007 (+) 624 WP_075621671.1 major tail protein -
  BSA145_RS03825 (BSA145_03760) gpG 719007..719378 (+) 372 WP_075621672.1 phage tail assembly chaperone G -
  BSA145_RS03830 (BSA145_03765) - 719587..723810 (+) 4224 WP_075621673.1 phage tail tape measure protein -
  BSA145_RS22545 - 723810..723974 (+) 165 WP_231120797.1 hypothetical protein -
  BSA145_RS03835 (BSA145_03770) - 723982..724611 (+) 630 WP_231120762.1 phage tail domain-containing protein -
  BSA145_RS03840 (BSA145_03775) - 724614..726494 (+) 1881 WP_075621674.1 phage tail spike protein -
  BSA145_RS03845 (BSA145_03780) - 726507..726728 (+) 222 WP_075621675.1 hypothetical protein -
  BSA145_RS03850 (BSA145_03785) - 726764..728371 (+) 1608 WP_075621676.1 right-handed parallel beta-helix repeat-containing protein -
  BSA145_RS03855 (BSA145_03790) - 728383..729987 (+) 1605 WP_075621677.1 BppU family phage baseplate upper protein -
  BSA145_RS03860 (BSA145_03795) - 730090..730290 (+) 201 WP_231120695.1 hypothetical protein -
  BSA145_RS03865 - 730287..730427 (+) 141 WP_075621679.1 XkdX family protein -
  BSA145_RS03870 (BSA145_03800) - 730467..730682 (+) 216 WP_075623626.1 BhlA/UviB family holin-like peptide -
  BSA145_RS03875 (BSA145_03805) - 730700..730963 (+) 264 WP_075621680.1 phage holin -
  BSA145_RS03880 (BSA145_03810) - 731024..731845 (+) 822 WP_075621681.1 N-acetylmuramoyl-L-alanine amidase -
  BSA145_RS03885 (BSA145_03815) - 731930..732442 (-) 513 WP_075621682.1 hypothetical protein -
  BSA145_RS03895 (BSA145_03825) - 732853..733998 (+) 1146 Protein_754 Rap family tetratricopeptide repeat protein -
  BSA145_RS22000 - 733988..734125 (+) 138 WP_155762150.1 hypothetical protein -
  BSA145_RS22245 - 734173..734328 (-) 156 WP_196774868.1 Arm DNA-binding domain-containing protein -
  BSA145_RS03905 (BSA145_03830) - 734396..734584 (-) 189 WP_075621684.1 hypothetical protein -
  BSA145_RS03910 (BSA145_03835) comFA 734846..735835 (+) 990 WP_418202168.1 helicase-related protein Machinery gene
  BSA145_RS03915 (BSA145_03840) - 735884..736177 (+) 294 WP_024423623.1 late competence development ComFB family protein -
  BSA145_RS22250 (BSA145_03845) comFC 736204..736860 (+) 657 WP_231120697.1 ComF family protein Machinery gene

Sequence


Protein


Download         Length: 218 a.a.        Molecular weight: 24924.04 Da        Isoelectric Point: 9.9145

>NTDB_id=181358 BSA145_RS22250 WP_231120697.1 736204..736860(+) (comFC) [Bacillus safensis strain U14-5]
MPAFTWKSFLLLNDDRLCVACKDHLVKIERPICPACGRAQSNDQLCQDCNRWNERSDSTKILVKNRSVYTYNDLMKDVLA
RFKFRGDTALAELFKKDVQAAFKHSFCIKEPLLIPIPLSHERLKERGFNQSVIIASLLDKPILQPLVKIHQSKQSKKKKN
ERLDQKGLFQIKQTDAIVQRDIILVDDIYTTGATIYDAARILKDAGAKSVSSFTLIRS

Nucleotide


Download         Length: 657 bp        

>NTDB_id=181358 BSA145_RS22250 WP_231120697.1 736204..736860(+) (comFC) [Bacillus safensis strain U14-5]
GTGCCTGCTTTTACATGGAAATCATTCCTACTATTAAATGATGATCGCCTTTGTGTAGCATGTAAGGATCACTTAGTAAA
GATTGAGCGTCCTATTTGTCCAGCATGCGGAAGGGCGCAGTCAAATGACCAATTGTGCCAGGACTGTAACAGATGGAATG
AACGCTCTGATTCTACTAAAATCCTTGTAAAAAATAGGTCAGTTTATACTTACAATGATTTGATGAAAGATGTCCTTGCC
CGCTTTAAATTTCGGGGTGATACGGCTTTAGCTGAGCTGTTTAAAAAAGATGTACAAGCAGCATTTAAACATTCCTTCTG
CATAAAAGAACCATTGCTGATACCGATTCCTTTAAGTCATGAAAGACTAAAAGAAAGAGGGTTTAATCAGTCCGTGATAA
TCGCTTCTTTGCTAGATAAGCCAATATTGCAACCACTCGTGAAAATACATCAAAGTAAGCAGTCTAAAAAAAAGAAAAAT
GAACGCCTAGATCAAAAAGGATTGTTTCAAATCAAGCAAACAGATGCGATTGTTCAAAGGGATATTATATTAGTTGATGA
TATCTATACAACGGGAGCAACCATCTATGATGCGGCAAGAATTTTGAAAGACGCAGGTGCTAAAAGTGTTTCTTCTTTTA
CGTTGATACGTAGTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFC Bacillus subtilis subsp. subtilis str. 168

50.463

99.083

0.5

  comFC/cflB Streptococcus pneumoniae Rx1

37.209

98.624

0.367

  comFC/cflB Streptococcus pneumoniae D39

37.209

98.624

0.367

  comFC/cflB Streptococcus pneumoniae R6

37.209

98.624

0.367

  comFC/cflB Streptococcus pneumoniae TIGR4

37.209

98.624

0.367


Multiple sequence alignment