Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BSA145_RS20360 Genome accession   NZ_CP015607
Coordinates   3892555..3893640 (-) Length   361 a.a.
NCBI ID   WP_075623498.1    Uniprot ID   A0A1L6ZN39
Organism   Bacillus safensis strain U14-5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3887555..3898640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSA145_RS20310 (BSA145_19875) sipW 3887649..3888221 (-) 573 WP_075623494.1 signal peptidase I SipW -
  BSA145_RS20315 (BSA145_19880) tapA 3888274..3888801 (-) 528 WP_075623495.1 amyloid fiber anchoring/assembly protein TapA -
  BSA145_RS20320 (BSA145_19885) - 3889102..3889380 (+) 279 WP_226014770.1 DUF3889 domain-containing protein -
  BSA145_RS20325 (BSA145_19890) - 3889415..3889609 (-) 195 WP_075623734.1 YqzE family protein -
  BSA145_RS20330 (BSA145_19895) - 3889669..3890052 (-) 384 WP_075623496.1 competence type IV pilus minor pilin ComGG -
  BSA145_RS20335 (BSA145_19900) comGF 3890049..3890576 (-) 528 WP_257787572.1 competence type IV pilus minor pilin ComGF -
  BSA145_RS23120 (BSA145_19905) comGE 3890488..3890802 (-) 315 WP_041106865.1 competence type IV pilus minor pilin ComGE -
  BSA145_RS20345 (BSA145_19910) comGD 3890786..3891232 (-) 447 WP_075623497.1 competence type IV pilus minor pilin ComGD -
  BSA145_RS20350 (BSA145_19915) comGC 3891225..3891518 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  BSA145_RS22955 comGB 3891535..3891816 (-) 282 WP_231120659.1 type II secretion system F family protein Machinery gene
  BSA145_RS20355 (BSA145_19920) - 3891828..3892574 (-) 747 WP_231120660.1 type II secretion system F family protein -
  BSA145_RS20360 (BSA145_19925) comGA 3892555..3893640 (-) 1086 WP_075623498.1 competence type IV pilus ATPase ComGA Machinery gene
  BSA145_RS20365 (BSA145_19930) - 3893818..3894693 (-) 876 WP_024422979.1 STAS domain-containing protein -
  BSA145_RS20375 (BSA145_19940) - 3894917..3895297 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BSA145_RS20380 (BSA145_19945) - 3895502..3895744 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  BSA145_RS20385 (BSA145_19950) - 3895855..3897210 (-) 1356 WP_075623499.1 IS1182 family transposase -
  BSA145_RS20390 (BSA145_19955) - 3897321..3897959 (-) 639 WP_041106873.1 MBL fold metallo-hydrolase -
  BSA145_RS20395 - 3898117..3898290 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 40934.49 Da        Isoelectric Point: 9.2439

>NTDB_id=181355 BSA145_RS20360 WP_075623498.1 3892555..3893640(-) (comGA) [Bacillus safensis strain U14-5]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRNGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLV
LSTLHAKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELICPFCGETCQLYCKLNRPFRRTNVFELLFGKELGECMKE
AKGEYAHSSYETLQKLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=181355 BSA145_RS20360 WP_075623498.1 3892555..3893640(-) (comGA) [Bacillus safensis strain U14-5]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCAGTGTCATTTCGTGTTGATTCAGATTTAATCCAGCAGCGAACCATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAACGGTCAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACGACAACACTATATTCATTGATCCAGTTT
GCAAAAAAGAATTTTAACCGAAATATTATTACCCTTAATATTATTACCCTTGAAGATCCTGTCGAAACAAGGAATGAAGA
AGTGCTGCAGGTTCAAGTGAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATTTTAAGACATGATCCAG
ATGTGATTGTGCTAGGAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTA
TTGAGTACGCTCCATGCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTCACGATGAATGAACTCGA
ACAAACCATGGTTGCGATTGCTGCCCAGCGATTAATTGAGCTCATATGTCCTTTTTGCGGAGAAACATGTCAGCTTTACT
GCAAATTAAATCGGCCTTTCAGACGAACAAATGTATTTGAATTGCTTTTCGGAAAAGAGCTTGGTGAGTGTATGAAAGAG
GCGAAAGGAGAATATGCACATTCGTCATATGAAACATTGCAAAAATTAATTCGTAAAGGAGTGGCACTAGGCTATTTATC
GAAAAACACATATCACCGCTGGGTTTATGAAGAAGCGAGTCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1L6ZN39

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

63.51

99.446

0.632

Multiple sequence alignment