Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   A4214_RS02075 Genome accession   NZ_CP015564
Coordinates   419741..421075 (+) Length   444 a.a.
NCBI ID   WP_015702713.1    Uniprot ID   A0AAW8VA23
Organism   Pasteurella multocida strain USDA-ARS-USMARC-60713     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 414741..426075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4214_RS02050 (A4214_02045) - 414818..417378 (-) 2561 Protein_393 penicillin-binding protein 1A -
  A4214_RS02055 (A4214_02050) - 417514..418287 (+) 774 WP_015702710.1 pilus assembly protein PilM -
  A4214_RS02060 (A4214_02055) - 418327..418842 (+) 516 WP_015702711.1 hypothetical protein -
  A4214_RS02065 (A4214_02060) - 418842..419366 (+) 525 WP_005751903.1 hypothetical protein -
  A4214_RS02070 (A4214_02065) - 419369..419731 (+) 363 WP_015702712.1 pilus assembly protein PilP -
  A4214_RS02075 (A4214_02070) comE 419741..421075 (+) 1335 WP_015702713.1 type IV pilus secretin PilQ Machinery gene
  A4214_RS02080 (A4214_02075) aroK 421258..421785 (+) 528 WP_005717623.1 shikimate kinase AroK -
  A4214_RS02085 (A4214_02080) aroB 421802..422890 (+) 1089 WP_005754750.1 3-dehydroquinate synthase -
  A4214_RS02090 (A4214_02085) - 422894..423799 (+) 906 WP_005723696.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  A4214_RS02095 (A4214_02090) - 423846..424151 (-) 306 WP_005723691.1 DUF721 domain-containing protein -
  A4214_RS02100 (A4214_02095) secM 424244..424555 (+) 312 WP_005723689.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49246.60 Da        Isoelectric Point: 8.0315

>NTDB_id=180842 A4214_RS02075 WP_015702713.1 419741..421075(+) (comE) [Pasteurella multocida strain USDA-ARS-USMARC-60713]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IARMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
TPVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNSILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKSSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=180842 A4214_RS02075 WP_015702713.1 419741..421075(+) (comE) [Pasteurella multocida strain USDA-ARS-USMARC-60713]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAGAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCTGCAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCGGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
ACACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTGGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACTCGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACTCCTCATATTATCAAATCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCAAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)

Bacterial type II and III secretion system protein (Secretin)

(264-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.269

97.297

0.606
  comE Haemophilus influenzae 86-028NP

61.574

97.297

0.599
  comE Glaesserella parasuis strain SC1401

49.192

97.523

0.48
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.563

92.793

0.367
  pilQ Vibrio cholerae strain A1552

39.563

92.793

0.367

Multiple sequence alignment