Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   BXO92_RS23185 Genome accession   NZ_CP019455
Coordinates   4548266..4549276 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain FHI_NMBU_03     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4543266..4554276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BXO92_RS23165 (BXO92_23455) znuA 4544066..4544998 (-) 933 WP_024223805.1 zinc ABC transporter substrate-binding protein ZnuA -
  BXO92_RS23170 (BXO92_23460) znuC 4545077..4545832 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  BXO92_RS23175 (BXO92_23465) znuB 4545829..4546614 (+) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  BXO92_RS23185 (BXO92_23485) ruvB 4548266..4549276 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  BXO92_RS23190 (BXO92_23490) ruvA 4549285..4549896 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  BXO92_RS23195 (BXO92_23495) yobI 4550035..4550100 (-) 66 WP_072093883.1 stress response small protein YobI -
  BXO92_RS23200 (BXO92_23500) yebB 4550171..4550773 (+) 603 WP_001024913.1 YebB family permuted papain-like enzyme -
  BXO92_RS23205 (BXO92_23505) ruvC 4550775..4551296 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  BXO92_RS23210 (BXO92_23510) yebC 4551331..4552071 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  BXO92_RS23215 (BXO92_23515) nudB 4552100..4552552 (-) 453 WP_077393227.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=180589 BXO92_RS23185 WP_000568519.1 4548266..4549276(-) (ruvB) [Escherichia coli strain FHI_NMBU_03]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=180589 BXO92_RS23185 WP_000568519.1 4548266..4549276(-) (ruvB) [Escherichia coli strain FHI_NMBU_03]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCTGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment