Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   A4W82_RS02760 Genome accession   NZ_CP015487
Coordinates   552922..554259 (+) Length   445 a.a.
NCBI ID   WP_052039239.1    Uniprot ID   A0AAE8J6D0
Organism   Latilactobacillus sakei strain TMW 1.46     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 547922..559259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W82_RS02745 (A4W82_02710) rny 549359..550924 (+) 1566 WP_011374197.1 ribonuclease Y -
  A4W82_RS02750 (A4W82_02715) - 551080..552168 (+) 1089 WP_035144637.1 MraY family glycosyltransferase -
  A4W82_RS02755 (A4W82_02720) - 552195..552860 (-) 666 WP_025016329.1 YigZ family protein -
  A4W82_RS02760 (A4W82_02725) comFA 552922..554259 (+) 1338 WP_052039239.1 DEAD/DEAH box helicase Machinery gene
  A4W82_RS02765 comFC 554502..554930 (+) 429 WP_151384770.1 ComF family protein Machinery gene
  A4W82_RS02770 (A4W82_02735) raiA 555057..555602 (+) 546 WP_011374202.1 ribosome-associated translation inhibitor RaiA -
  A4W82_RS02775 (A4W82_02740) secA 555851..558214 (+) 2364 WP_011374203.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 50353.78 Da        Isoelectric Point: 9.7862

>NTDB_id=179978 A4W82_RS02760 WP_052039239.1 552922..554259(+) (comFA) [Latilactobacillus sakei strain TMW 1.46]
MGQQVIACGRQFTAAQLADTQNNNYSLPQIKRRPAFLRVKHRLVCQRCQQVVPPQTCLPDGRHYCAQCLLFGRLVEGDWL
YTVPECHLFENNIPKLTWEGQLTPHQEQAAQAVVEVVQTQKRHVLTAVTGAGKTEMLFQGILVALQKGQRVCLAAPRVAV
CLELYPRLQAAFATASIMLMHGEQTEPYRYTQLVICTTHQLLKFYHAFDTVIVDEVDAFPFVDNPVLVTAVEQACKPQCA
LLYLTATPTPAIKRAIAAKQMTVSELPLRFHGHLLPEPHRHVAFNWRAQLKKGRLPKRLERDCQTCLKTQQILLFVPQVR
LLQPVANRLSTLLPAVRIETLHANDPDQIKKITAFRAQQIQLMVTTTILERGVTFKNVAVLVLGAEHMVFNEAVLVQIAG
RAGRHKDYAQNPVHFYYQDYTRAIKGACRQIKAQNQRGRRLQSTV

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=179978 A4W82_RS02760 WP_052039239.1 552922..554259(+) (comFA) [Latilactobacillus sakei strain TMW 1.46]
ATGGGACAACAAGTAATTGCCTGTGGTCGCCAGTTTACGGCGGCCCAGTTAGCCGATACGCAAAATAATAACTATAGTTT
ACCACAGATTAAGCGGCGACCTGCGTTTTTACGGGTCAAGCATCGCCTAGTCTGCCAACGTTGTCAGCAAGTGGTGCCGC
CGCAAACCTGCTTACCAGATGGGCGCCATTATTGCGCGCAATGTTTGTTATTTGGTCGTTTGGTAGAGGGCGATTGGTTA
TACACGGTACCGGAGTGTCATTTATTCGAAAATAACATACCAAAGTTGACGTGGGAGGGGCAATTAACACCGCATCAGGA
ACAAGCTGCCCAAGCGGTGGTTGAGGTGGTTCAAACACAAAAACGGCACGTATTAACGGCCGTGACCGGCGCAGGGAAGA
CCGAGATGCTTTTTCAAGGAATTTTAGTTGCGCTTCAAAAGGGTCAGCGGGTATGTCTGGCAGCGCCTAGAGTCGCCGTT
TGCTTGGAACTTTATCCGCGCCTGCAAGCTGCCTTTGCAACGGCTAGTATCATGTTGATGCACGGTGAGCAAACTGAGCC
CTATCGCTATACGCAGTTAGTGATTTGCACGACGCATCAGTTGCTGAAGTTTTATCACGCCTTTGACACTGTGATTGTCG
ATGAAGTCGATGCTTTTCCTTTTGTTGATAATCCCGTTTTAGTCACGGCAGTTGAACAAGCCTGCAAGCCCCAATGTGCT
TTATTATATTTAACGGCGACTCCGACCCCCGCTATTAAACGGGCAATTGCTGCTAAACAAATGACGGTTAGCGAATTACC
GCTCCGCTTTCATGGTCATCTATTACCAGAACCACATAGGCACGTCGCTTTTAATTGGCGGGCCCAATTAAAAAAAGGTC
GGTTACCTAAACGACTTGAGCGGGATTGCCAGACTTGTTTAAAAACACAACAGATTTTGTTATTCGTGCCGCAAGTGCGG
TTATTGCAACCAGTTGCCAATCGGTTATCAACCTTATTGCCGGCGGTCAGGATTGAAACATTGCATGCTAACGATCCGGA
TCAGATCAAAAAGATTACGGCGTTTAGAGCACAACAAATTCAGCTAATGGTGACGACCACCATTTTAGAACGGGGCGTTA
CGTTTAAAAACGTTGCAGTTTTGGTCTTAGGCGCGGAACACATGGTGTTTAACGAAGCCGTTTTGGTCCAGATTGCCGGG
CGAGCGGGACGGCATAAAGATTACGCCCAAAATCCGGTTCATTTTTATTATCAAGACTATACGCGGGCGATCAAAGGCGC
TTGTCGGCAAATTAAGGCCCAAAATCAGAGGGGACGGCGATTACAATCAACTGTTTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(307-405)

Type III restriction enzyme, res subunit (ResIII)

(101-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Latilactobacillus sakei subsp. sakei 23K

98.652

100

0.987
  comFA/cflA Streptococcus pneumoniae TIGR4

38.979

96.854

0.378
  comFA/cflA Streptococcus pneumoniae D39

38.747

96.854

0.375
  comFA/cflA Streptococcus pneumoniae R6

38.747

96.854

0.375
  comFA/cflA Streptococcus pneumoniae Rx1

38.747

96.854

0.375
  comFA/cflA Streptococcus mitis SK321

38.515

96.854

0.373
  comFA/cflA Streptococcus mitis NCTC 12261

38.283

96.854

0.371
  comFA Bacillus subtilis subsp. subtilis str. 168

39.268

92.135

0.362

Multiple sequence alignment