Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   A1D33_RS16750 Genome accession   NZ_CP015443
Coordinates   3526545..3527615 (+) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain CC09     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3521545..3532615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1D33_RS16720 (A1D33_016715) - 3522018..3522263 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  A1D33_RS16725 (A1D33_016720) - 3522500..3522880 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  A1D33_RS16735 (A1D33_016730) - 3523087..3523917 (+) 831 WP_039254482.1 STAS domain-containing protein -
  A1D33_RS16740 (A1D33_016735) - 3523955..3525256 (-) 1302 WP_012117986.1 hemolysin family protein -
  A1D33_RS16745 (A1D33_016740) - 3525402..3526352 (+) 951 WP_015417820.1 magnesium transporter CorA family protein -
  A1D33_RS16750 (A1D33_016745) comGA 3526545..3527615 (+) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  A1D33_RS16755 (A1D33_016750) comGB 3527602..3528639 (+) 1038 WP_015417819.1 competence type IV pilus assembly protein ComGB Machinery gene
  A1D33_RS16760 (A1D33_016755) comGC 3528686..3528952 (+) 267 WP_050515801.1 competence type IV pilus major pilin ComGC Machinery gene
  A1D33_RS16765 (A1D33_016760) comGD 3528942..3529379 (+) 438 WP_015417817.1 competence type IV pilus minor pilin ComGD Machinery gene
  A1D33_RS16770 (A1D33_016765) comGE 3529363..3529677 (+) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  A1D33_RS16775 (A1D33_016770) comGF 3529586..3530086 (+) 501 WP_257738552.1 competence type IV pilus minor pilin ComGF -
  A1D33_RS16780 (A1D33_016775) comGG 3530087..3530464 (+) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  A1D33_RS16785 (A1D33_016780) - 3530521..3530700 (+) 180 WP_003153093.1 YqzE family protein -
  A1D33_RS16790 (A1D33_016785) - 3530740..3531069 (-) 330 WP_039254490.1 DUF3889 domain-containing protein -
  A1D33_RS16795 (A1D33_016790) tapA 3531328..3531999 (+) 672 WP_031378945.1 amyloid fiber anchoring/assembly protein TapA -
  A1D33_RS16800 (A1D33_016795) sipW 3531971..3532555 (+) 585 WP_015240205.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=179878 A1D33_RS16750 WP_003153083.1 3526545..3527615(+) (comGA) [Bacillus velezensis strain CC09]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=179878 A1D33_RS16750 WP_003153083.1 3526545..3527615(+) (comGA) [Bacillus velezensis strain CC09]
GTGAAAAATATTGAAACATATAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATTTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment