Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   BWI89_RS04660 Genome accession   NZ_CP019273
Coordinates   910128..911138 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain 13P477T     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 905128..916138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWI89_RS04630 (BWI89_04630) nudB 906852..907304 (+) 453 WP_001308712.1 dihydroneopterin triphosphate diphosphatase -
  BWI89_RS04635 (BWI89_04635) yebC 907333..908073 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  BWI89_RS04640 (BWI89_04640) ruvC 908108..908629 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  BWI89_RS04645 (BWI89_04645) yebB 908631..909233 (-) 603 WP_001024940.1 YebB family permuted papain-like enzyme -
  BWI89_RS04650 (BWI89_04650) yobI 909304..909369 (+) 66 WP_072123646.1 stress response small protein YobI -
  BWI89_RS04655 (BWI89_04655) ruvA 909508..910119 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  BWI89_RS04660 (BWI89_04660) ruvB 910128..911138 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  BWI89_RS04665 (BWI89_04665) znuB 911283..912068 (-) 786 WP_000571474.1 zinc ABC transporter permease subunit ZnuB -
  BWI89_RS04670 (BWI89_04670) znuC 912065..912820 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  BWI89_RS04675 (BWI89_04675) znuA 912899..913831 (+) 933 WP_024186182.1 zinc ABC transporter substrate-binding protein ZnuA -
  BWI89_RS04680 (BWI89_04680) mepM 913847..915169 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=179568 BWI89_RS04660 WP_000568519.1 910128..911138(+) (ruvB) [Escherichia coli strain 13P477T]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=179568 BWI89_RS04660 WP_000568519.1 910128..911138(+) (ruvB) [Escherichia coli strain 13P477T]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCTGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment