Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   A6037_RS00860 Genome accession   NZ_CP015425
Coordinates   201238..201684 (-) Length   148 a.a.
NCBI ID   WP_064081943.1    Uniprot ID   -
Organism   [Haemophilus] ducreyi strain VAN2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 196238..206684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6037_RS00835 (A6037_00850) yacG 197184..197372 (-) 189 WP_064081939.1 DNA gyrase inhibitor YacG -
  A6037_RS00840 (A6037_00855) coaE 197375..197992 (-) 618 WP_064081940.1 dephospho-CoA kinase -
  A6037_RS07710 (A6037_00860) - 198010..198324 (-) 315 WP_158077649.1 prepilin peptidase -
  A6037_RS07715 (A6037_00865) - 198386..198655 (-) 270 WP_078179334.1 hypothetical protein -
  A6037_RS00850 (A6037_00870) pilC 198655..199848 (-) 1194 WP_064081941.1 type II secretion system F family protein Machinery gene
  A6037_RS00855 (A6037_00875) pilB 199832..201226 (-) 1395 WP_064081942.1 GspE/PulE family protein Machinery gene
  A6037_RS00860 (A6037_00880) pilA 201238..201684 (-) 447 WP_064081943.1 pilin Machinery gene
  A6037_RS00865 (A6037_00885) radA 201878..203257 (+) 1380 WP_064081944.1 DNA repair protein RadA -
  A6037_RS00870 (A6037_00890) - 203324..203539 (-) 216 WP_010945033.1 YgjV family protein -
  A6037_RS00875 (A6037_00895) - 203661..204146 (-) 486 WP_064081945.1 dihydrofolate reductase -
  A6037_RS00880 (A6037_00900) - 204267..205037 (+) 771 WP_064082999.1 TSUP family transporter -
  A6037_RS00885 (A6037_00905) nth 205046..205681 (+) 636 WP_010945029.1 endonuclease III -
  A6037_RS00890 (A6037_00910) fdhD 205741..206568 (-) 828 WP_064081946.1 formate dehydrogenase accessory sulfurtransferase FdhD -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15856.17 Da        Isoelectric Point: 7.5799

>NTDB_id=179437 A6037_RS00860 WP_064081943.1 201238..201684(-) (pilA) [[Haemophilus] ducreyi strain VAN2]
MSSLFLIKPLQKAFTLIELMIVIAIIAILATVAIPSYNSYTKKAALSELLAASSAYKTDVEICVYNLGRTDGCNAGTNGI
KNDNSGKSKYVGSISVENGTITVTGKESLKDYSYKMTPTLQGESLTWKTTCNGADTSLFPANFCSTEQ

Nucleotide


Download         Length: 447 bp        

>NTDB_id=179437 A6037_RS00860 WP_064081943.1 201238..201684(-) (pilA) [[Haemophilus] ducreyi strain VAN2]
ATGTCATCTTTATTTTTGATTAAGCCACTACAAAAGGCGTTTACTTTAATTGAATTAATGATTGTGATTGCCATTATCGC
AATTTTAGCTACGGTTGCGATTCCTTCTTACAATAGTTATACCAAAAAAGCTGCGTTATCTGAGTTATTAGCCGCTTCAT
CAGCGTATAAAACCGATGTAGAAATATGTGTTTATAATTTGGGTAGAACAGATGGATGTAATGCAGGAACAAACGGTATA
AAAAATGATAATTCAGGTAAAAGTAAATATGTCGGATCAATCAGTGTAGAAAATGGAACGATTACAGTAACGGGTAAAGA
AAGCCTTAAAGATTATAGTTATAAAATGACACCAACCTTACAAGGCGAATCCTTAACTTGGAAAACGACTTGTAATGGTG
CTGATACTAGCCTTTTCCCTGCTAATTTTTGTTCTACTGAACAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(11-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

61.486

100

0.615
  pilA Haemophilus influenzae 86-028NP

57.246

93.243

0.534
  pilA Haemophilus influenzae Rd KW20

54.167

97.297

0.527

Multiple sequence alignment