Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   BWI86_RS06075 Genome accession   NZ_CP019259
Coordinates   1187993..1188652 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain 13C1065T     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1182993..1193652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWI86_RS06045 (BWI86_06060) parE 1183084..1184976 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  BWI86_RS06050 (BWI86_06065) ygiN 1185024..1185338 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  BWI86_RS06055 (BWI86_06070) mdaB 1185369..1185950 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  BWI86_RS06065 (BWI86_06080) ygiZ 1186269..1186601 (+) 333 WP_000917684.1 DUF2645 family protein -
  BWI86_RS06070 (BWI86_06085) qseC 1186647..1187996 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  BWI86_RS06075 (BWI86_06090) ciaR 1187993..1188652 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  BWI86_RS06080 (BWI86_06095) ygiW 1188804..1189196 (+) 393 WP_148116247.1 OB fold stress tolerance protein YgiW -
  BWI86_RS06085 (BWI86_06100) ygiV 1189249..1189731 (+) 483 WP_000183505.1 AraC family transcriptional regulator -
  BWI86_RS06090 (BWI86_06105) mqsR 1189936..1190232 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  BWI86_RS06095 (BWI86_06110) mqsA 1190234..1190629 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  BWI86_RS06105 (BWI86_06115) ygiS 1190762..1192369 (+) 1608 WP_148116248.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=179345 BWI86_RS06075 WP_001221493.1 1187993..1188652(-) (ciaR) [Escherichia coli strain 13C1065T]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=179345 BWI86_RS06075 WP_001221493.1 1187993..1188652(-) (ciaR) [Escherichia coli strain 13C1065T]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment