Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   BWI82_RS03620 Genome accession   NZ_CP019243
Coordinates   668848..669858 (-) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain Combat2C1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 663848..674858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWI82_RS03600 (BWI82_03585) mepM 664814..666136 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  BWI82_RS03605 (BWI82_03590) znuA 666152..667084 (-) 933 WP_001339711.1 zinc ABC transporter substrate-binding protein ZnuA -
  BWI82_RS03610 (BWI82_03595) znuC 667163..667918 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  BWI82_RS03615 (BWI82_03600) znuB 667915..668700 (+) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  BWI82_RS03620 (BWI82_03605) ruvB 668848..669858 (-) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  BWI82_RS03625 (BWI82_03610) ruvA 669867..670479 (-) 613 Protein_683 Holliday junction branch migration protein RuvA -
  BWI82_RS03630 (BWI82_03615) yobI 670618..670683 (-) 66 WP_072123646.1 stress response small protein YobI -
  BWI82_RS03635 (BWI82_03620) yebB 670757..671359 (+) 603 WP_001024942.1 YebB family permuted papain-like enzyme -
  BWI82_RS03640 (BWI82_03625) ruvC 671361..671882 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  BWI82_RS03645 (BWI82_03630) yebC 671917..672657 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  BWI82_RS03650 (BWI82_03635) nudB 672686..673138 (-) 453 WP_077393227.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=178992 BWI82_RS03620 WP_000568522.1 668848..669858(-) (ruvB) [Escherichia coli strain Combat2C1]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=178992 BWI82_RS03620 WP_000568522.1 668848..669858(-) (ruvB) [Escherichia coli strain Combat2C1]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494