Detailed information NTG0178845

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Aba3207_RS15855
• Genome accession: NZ_CP015364
• Coordinates: 3269156..3270274 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347041.1
• Uniprot ID: A0A3R9E3W9
• Organism: Acinetobacter baumannii strain 3207
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3264156..3275274

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Aba3207_RS15825 (Aba3207_15825)	-	3264985..3265833 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
Aba3207_RS15830 (Aba3207_15830)	-	3266086..3266541 (+)	456	WP_000782976.1	bacteriohemerythrin	-
Aba3207_RS15835 (Aba3207_15835)	-	3266694..3267761 (+)	1068	WP_029642282.1	membrane protein	-
Aba3207_RS15840 (Aba3207_15840)	-	3267761..3268084 (+)	324	WP_000442585.1	RnfH family protein	-
Aba3207_RS15845 (Aba3207_15845)	-	3268139..3268537 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
Aba3207_RS15850 (Aba3207_15850)	fur	3268649..3269086 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
Aba3207_RS15855 (Aba3207_15855)	pilU	3269156..3270274 (-)	1119	WP_000347041.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Aba3207_RS15860 (Aba3207_15860)	pilT	3270302..3271339 (-)	1038	WP_000355487.1	type IV pilus twitching motility protein PilT	Machinery gene
Aba3207_RS15865 (Aba3207_15865)	-	3271466..3272158 (+)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41922.14 Da
• Isoelectric Point: 6.5789

>NTDB_id=178845 Aba3207_RS15855 WP_000347041.1 3269156..3270274(-) (pilU) [Acinetobacter baumannii strain 3207]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQYLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=178845 Aba3207_RS15855 WP_000347041.1 3269156..3270274(-) (pilU) [Acinetobacter baumannii strain 3207]
ATGGATTTTAATGACTTGCTCAATCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGTATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

3D structure

• AlphaFold DB: A0A3R9E3W9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	68.194	99.731	0.68
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392