Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   I596_RS09850 Genome accession   NZ_CP015249
Coordinates   2416844..2417902 (-) Length   352 a.a.
NCBI ID   WP_067647133.1    Uniprot ID   A0A160DW90
Organism   Dokdonella koreensis DS-123     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2411844..2422902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I596_RS09830 (I596_2110) pilQ 2412530..2414803 (-) 2274 WP_223303806.1 type IV pilus secretin PilQ -
  I596_RS09835 (I596_2111) - 2414826..2415359 (-) 534 WP_067647125.1 pilus assembly protein PilP -
  I596_RS09840 (I596_2112) pilO 2415361..2416083 (-) 723 WP_067647128.1 type 4a pilus biogenesis protein PilO -
  I596_RS09845 (I596_2113) - 2416080..2416844 (-) 765 WP_067647130.1 PilN domain-containing protein -
  I596_RS09850 (I596_2114) pilM 2416844..2417902 (-) 1059 WP_067647133.1 pilus assembly protein PilM Machinery gene
  I596_RS09855 (I596_2115) - 2418140..2420617 (+) 2478 WP_067647136.1 penicillin-binding protein 1A -
  I596_RS09860 (I596_2116) - 2420645..2421280 (+) 636 WP_067647139.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37803.36 Da        Isoelectric Point: 4.3757

>NTDB_id=178039 I596_RS09850 WP_067647133.1 2416844..2417902(-) (pilM) [Dokdonella koreensis DS-123]
MGLFTAKTPALIGVDISSTAVKLLQLGQVGGRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEALRRALNRSGAKTRSAAAA
VAGSAVITKIIPMPADLSEDDMEAQIQVEANQYIPYPIEEVSLDFEVVGPVKDNPDMVNVLLAASRTENVDLRVAALDLG
GLSARVMDVEAFAMENAYRLITDQLSVSKDAVVAVVDVGATMTTLIVLRNQRTIYSREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYEVEVLEPFKEAMVQQISRLLQFFFAGSEYSRVDQVVLAGGCASIAGVSDLIEEQLGVPCVVANPLS
NMALSNRVQAQALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=178039 I596_RS09850 WP_067647133.1 2416844..2417902(-) (pilM) [Dokdonella koreensis DS-123]
GTGGGCTTGTTCACTGCGAAAACACCGGCCCTGATCGGTGTGGACATCAGTTCGACCGCGGTCAAGTTGCTACAGCTGGG
GCAGGTTGGGGGACGTTATCGCGTAGAGCACTACGCGGTCGAGCCGCTGCCGCCCAACGCCGTCGTCGAAAAGAACATCG
TCGAGGTCGAGGCGGTCGGGGAAGCCCTGCGCCGCGCGCTGAACCGTTCAGGCGCCAAGACGCGCAGCGCGGCGGCCGCC
GTCGCGGGGTCCGCGGTGATCACCAAGATCATCCCGATGCCGGCCGACCTCAGCGAAGACGACATGGAAGCGCAGATCCA
GGTCGAGGCCAACCAGTACATTCCGTACCCGATCGAGGAGGTCAGCCTCGATTTCGAGGTGGTCGGCCCGGTCAAGGACA
ACCCGGACATGGTCAATGTCCTGCTGGCCGCCTCGCGGACCGAGAACGTCGACCTGCGCGTGGCCGCGCTCGACCTCGGC
GGCCTGAGCGCCCGGGTGATGGACGTGGAGGCGTTCGCGATGGAGAACGCCTATCGCCTGATCACCGACCAGCTCTCCGT
ATCCAAGGATGCCGTGGTCGCCGTCGTCGACGTCGGCGCGACGATGACGACCCTGATCGTGCTGCGCAACCAGCGCACGA
TCTATTCGCGCGAGCAGGTCTTCGGCGGCAAGCAGCTGACCGACGAGGTCATGCGCCGCTACGGCCTTTCCTATGAGGAA
GCGGGCCTCGCCAAGCGCCAGGGCGGCCTGCCCGAGTCCTACGAGGTCGAGGTGCTGGAACCGTTCAAGGAGGCGATGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTTCGCCGGCAGCGAGTACAGCCGCGTCGACCAGGTGGTGCTGGCCGGGG
GATGCGCGTCGATCGCCGGCGTCTCGGACCTGATCGAGGAACAGCTCGGCGTGCCGTGCGTGGTCGCCAACCCGTTGTCG
AACATGGCGCTGTCCAACCGGGTCCAGGCGCAGGCGCTGGCCCAGGACGCGCCGGCCCTGATGATCGCTTGCGGCCTCGC
CCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A160DW90

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.989

100

0.52
  comM Acinetobacter nosocomialis M2

51.989

100

0.52
  comM Acinetobacter baylyi ADP1

51.143

99.432

0.509
  pilM Legionella pneumophila strain ERS1305867

42.776

100

0.429

Multiple sequence alignment