Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   A4265_RS00670 Genome accession   NZ_CP015238
Coordinates   113626..114060 (+) Length   144 a.a.
NCBI ID   WP_063631712.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NS53     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 108626..119060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4265_RS00640 (A4265_00550) - 110295..110660 (+) 366 WP_002986560.1 DUF1033 family protein -
  A4265_RS00645 (A4265_00555) comYA 110753..111691 (+) 939 WP_063629337.1 competence type IV pilus ATPase ComGA Machinery gene
  A4265_RS00650 (A4265_00560) comYB 111627..112661 (+) 1035 WP_227874845.1 competence type IV pilus assembly protein ComGB Machinery gene
  A4265_RS00655 (A4265_00565) comYC 112663..112989 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  A4265_RS00660 (A4265_00570) comGD 112964..113392 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  A4265_RS00665 (A4265_00575) comGE 113349..113633 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  A4265_RS00670 (A4265_00580) comYF 113626..114060 (+) 435 WP_063631712.1 competence type IV pilus minor pilin ComGF Machinery gene
  A4265_RS00675 (A4265_00585) comGG 114044..114370 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  A4265_RS00680 (A4265_00590) comYH 114468..115421 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  A4265_RS00685 (A4265_00595) - 115480..116676 (+) 1197 WP_063631713.1 acetate kinase -
  A4265_RS00690 (A4265_08495) - 116863..117171 (+) 309 Protein_114 hypothetical protein -
  A4265_RS00695 (A4265_00600) proC 117254..118024 (-) 771 WP_063631714.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16654.28 Da        Isoelectric Point: 10.2526

>NTDB_id=177879 A4265_RS00670 WP_063631712.1 113626..114060(+) (comYF) [Streptococcus pyogenes strain NS53]
MSKQLSNIKAFTLIEALIALLVISGSLLVYQGLTRTLLKHSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=177879 A4265_RS00670 WP_063631712.1 113626..114060(+) (comYF) [Streptococcus pyogenes strain NS53]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTATAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCGAACCCTCCTTAAACATAGCCATTATCTAGCCCGTCATGATCAAGATAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

50.36

96.528

0.486
  comYF Streptococcus mutans UA159

49.64

96.528

0.479
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

43.284

93.056

0.403
  comGF/cglF Streptococcus pneumoniae Rx1

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae D39

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae R6

42.105

92.361

0.389
  comGF/cglF Streptococcus pneumoniae TIGR4

42.105

92.361

0.389
  comGF/cglF Streptococcus mitis SK321

41.353

92.361

0.382

Multiple sequence alignment