Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   A4265_RS00650 Genome accession   NZ_CP015238
Coordinates   111627..112661 (+) Length   344 a.a.
NCBI ID   WP_227874845.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NS53     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 106627..117661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4265_RS00640 (A4265_00550) - 110295..110660 (+) 366 WP_002986560.1 DUF1033 family protein -
  A4265_RS00645 (A4265_00555) comYA 110753..111691 (+) 939 WP_063629337.1 competence type IV pilus ATPase ComGA Machinery gene
  A4265_RS00650 (A4265_00560) comYB 111627..112661 (+) 1035 WP_227874845.1 competence type IV pilus assembly protein ComGB Machinery gene
  A4265_RS00655 (A4265_00565) comYC 112663..112989 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  A4265_RS00660 (A4265_00570) comGD 112964..113392 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  A4265_RS00665 (A4265_00575) comGE 113349..113633 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  A4265_RS00670 (A4265_00580) comYF 113626..114060 (+) 435 WP_063631712.1 competence type IV pilus minor pilin ComGF Machinery gene
  A4265_RS00675 (A4265_00585) comGG 114044..114370 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  A4265_RS00680 (A4265_00590) comYH 114468..115421 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  A4265_RS00685 (A4265_00595) - 115480..116676 (+) 1197 WP_063631713.1 acetate kinase -
  A4265_RS00690 (A4265_08495) - 116863..117171 (+) 309 Protein_114 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39692.15 Da        Isoelectric Point: 9.7085

>NTDB_id=177877 A4265_RS00650 WP_227874845.1 111627..112661(+) (comYB) [Streptococcus pyogenes strain NS53]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDIMSIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=177877 A4265_RS00650 WP_227874845.1 111627..112661(+) (comYB) [Streptococcus pyogenes strain NS53]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGTCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.982

98.547

0.532
  comYB Streptococcus mutans UA159

53.982

98.547

0.532
  comYB Streptococcus gordonii str. Challis substr. CH1

50.588

98.837

0.5
  comGB/cglB Streptococcus mitis NCTC 12261

47.619

97.674

0.465
  comGB/cglB Streptococcus mitis SK321

47.748

96.802

0.462
  comGB/cglB Streptococcus pneumoniae Rx1

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae D39

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae R6

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae TIGR4

45.646

96.802

0.442
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404

Multiple sequence alignment