Detailed information NTG0177424

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: A4H00_RS03435
• Genome accession: NZ_CP015196
• Coordinates: 677337..677774 (-)
• Length: 145 a.a.
• NCBI ID: WP_067087309.1
• Uniprot ID: -
• Organism: Streptococcus marmotae strain HTS5
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 672337..682774

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A4H00_RS03410	pepA	672348..673421 (+)	1074	WP_067087297.1	glutamyl aminopeptidase	-
A4H00_RS03415	-	673461..674024 (-)	564	WP_067087300.1	folate family ECF transporter S component	-
A4H00_RS03420	-	674641..675831 (-)	1191	WP_067087302.1	acetate kinase	-
A4H00_RS03425	comYH	675880..676833 (-)	954	WP_067087305.1	class I SAM-dependent methyltransferase	Machinery gene
A4H00_RS03430	comGG	676856..677365 (-)	510	WP_067087307.1	competence type IV pilus minor pilin ComGG	-
A4H00_RS03435	comYF	677337..677774 (-)	438	WP_067087309.1	competence type IV pilus minor pilin ComGF	Machinery gene
A4H00_RS03440	comGE	677758..677997 (-)	240	WP_237334209.1	competence type IV pilus minor pilin ComGE	-
A4H00_RS03445	comGD/cglD	678023..678460 (-)	438	WP_067087315.1	competence type IV pilus minor pilin ComGD	Machinery gene
A4H00_RS03450	comGC/cglC	678411..678725 (-)	315	WP_067087318.1	competence type IV pilus major pilin ComGC	Machinery gene
A4H00_RS03455	comYB	678727..679755 (-)	1029	WP_167541367.1	competence type IV pilus assembly protein ComGB	Machinery gene
A4H00_RS03460	comGA/cglA/cilD	679676..680626 (-)	951	WP_067087321.1	competence type IV pilus ATPase ComGA	Machinery gene
A4H00_RS03465	-	681214..681525 (-)	312	WP_067087324.1	DUF1292 domain-containing protein	-
A4H00_RS03470	ruvX	681536..681955 (-)	420	WP_067087326.1	Holliday junction resolvase RuvX	-
A4H00_RS03475	-	681955..682221 (-)	267	WP_067087329.1	IreB family regulatory phosphoprotein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16901.30 Da
• Isoelectric Point: 7.1639

>NTDB_id=177424 A4H00_RS03435 WP_067087309.1 677337..677774(-) (comYF) [Streptococcus marmotae strain HTS5]
MWRKSKVAGFTLLECLVALLILSGGLLVFEGLTKLIHHEIAYQTTNVEKDWLVFVDQFRSELDGVRLVKVENNRLYVDKS
GQQLSFGKSKADDFRKTNDRGRGYQPMLYQVAAATVYQESQYVQIDFVFENGWERSFVYQFEEKK

Nucleotide

• Length: 438 bp

>NTDB_id=177424 A4H00_RS03435 WP_067087309.1 677337..677774(-) (comYF) [Streptococcus marmotae strain HTS5]
ATGTGGCGAAAGAGTAAGGTGGCAGGTTTTACCTTGTTAGAGTGTTTGGTTGCTCTGCTGATTTTATCTGGTGGATTACT
CGTTTTTGAGGGGCTGACGAAGCTGATTCATCATGAAATAGCCTATCAGACGACGAATGTAGAGAAGGACTGGCTTGTTT
TTGTAGACCAGTTTCGCTCGGAATTGGATGGGGTTCGGCTAGTCAAAGTGGAGAACAATCGCCTATATGTCGATAAATCA
GGGCAACAATTATCTTTCGGAAAGTCAAAGGCAGATGATTTTCGCAAGACAAACGATCGTGGTCGTGGTTACCAACCGAT
GCTGTATCAGGTAGCCGCTGCAACTGTCTACCAAGAAAGCCAGTATGTTCAGATTGATTTTGTATTTGAAAATGGTTGGG
AGAGAAGTTTTGTTTATCAATTTGAAGAAAAGAAGTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (39-134)

• Prokaryotic N-terminal methylation motif (N_methyl) (5-23)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	54.61	97.241	0.531
comYF	Streptococcus mutans UA159	53.901	97.241	0.524
comGF/cglF	Streptococcus pneumoniae D39	48.252	98.621	0.476
comGF/cglF	Streptococcus pneumoniae R6	48.252	98.621	0.476
comGF/cglF	Streptococcus pneumoniae TIGR4	48.252	98.621	0.476
comGF/cglF	Streptococcus pneumoniae Rx1	48.252	98.621	0.476
comGF	Lactococcus lactis subsp. cremoris KW2	47.183	97.931	0.462
comGF/cglF	Streptococcus mitis SK321	45.455	98.621	0.448
comGF/cglF	Streptococcus mitis NCTC 12261	45.455	98.621	0.448