Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   BE964_RS04325 Genome accession   NZ_CP018953
Coordinates   517991..519001 (-) Length   336 a.a.
NCBI ID   WP_000568520.1    Uniprot ID   -
Organism   Escherichia coli strain Ecol_276     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 512991..524001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BE964_RS04295 (BE964_04390) znuA 513805..514737 (-) 933 WP_001347089.1 zinc ABC transporter substrate-binding protein ZnuA -
  BE964_RS04300 (BE964_04395) znuC 514816..515571 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  BE964_RS04305 (BE964_04400) znuB 515568..516353 (+) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  BE964_RS27060 - 516547..517881 (-) 1335 Protein_515 IS3 family transposase -
  BE964_RS04325 (BE964_04420) ruvB 517991..519001 (-) 1011 WP_000568520.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  BE964_RS04330 (BE964_04425) ruvA 519010..519621 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  BE964_RS04335 (BE964_04430) yobI 519760..519825 (-) 66 WP_072093883.1 stress response small protein YobI -
  BE964_RS04340 (BE964_04435) yebB 519896..520498 (+) 603 WP_001024911.1 YebB family permuted papain-like enzyme -
  BE964_RS04345 (BE964_04440) ruvC 520500..521021 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  BE964_RS04350 (BE964_04445) yebC 521056..521796 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  BE964_RS04355 (BE964_04450) nudB 521825..522277 (-) 453 WP_077249130.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37163.73 Da        Isoelectric Point: 4.7818

>NTDB_id=177007 BE964_RS04325 WP_000568520.1 517991..519001(-) (ruvB) [Escherichia coli strain Ecol_276]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMS

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=177007 BE964_RS04325 WP_000568520.1 517991..519001(-) (ruvB) [Escherichia coli strain Ecol_276]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGCGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGTCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494