Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   A4W93_RS24650 Genome accession   NZ_CP015118
Coordinates   5390596..5392320 (+) Length   574 a.a.
NCBI ID   WP_085753130.1    Uniprot ID   A0A1W6LEY3
Organism   Piscinibacter gummiphilus strain NS21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5385596..5397320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W93_RS24635 (A4W93_24525) - 5386105..5387109 (-) 1005 WP_085753127.1 YeiH family protein -
  A4W93_RS24640 (A4W93_24530) - 5387106..5388728 (-) 1623 WP_085753128.1 3-(methylthio)propionyl-CoA ligase -
  A4W93_RS24645 (A4W93_24535) - 5388995..5390224 (-) 1230 WP_085753129.1 acyl-CoA dehydrogenase family protein -
  A4W93_RS24650 (A4W93_24540) pilF 5390596..5392320 (+) 1725 WP_085753130.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A4W93_RS24655 (A4W93_24545) pilC 5392422..5393639 (+) 1218 WP_085753131.1 type II secretion system F family protein Machinery gene
  A4W93_RS24660 (A4W93_24550) pilD 5393700..5394509 (+) 810 WP_269466971.1 A24 family peptidase Machinery gene
  A4W93_RS24665 (A4W93_24555) coaE 5394514..5395173 (+) 660 WP_085753133.1 dephospho-CoA kinase -
  A4W93_RS24670 (A4W93_24560) zapD 5395193..5395948 (+) 756 WP_085753134.1 cell division protein ZapD -
  A4W93_RS24675 (A4W93_24565) - 5395945..5396154 (+) 210 WP_085753135.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62592.88 Da        Isoelectric Point: 5.6231

>NTDB_id=176847 A4W93_RS24650 WP_085753130.1 5390596..5392320(+) (pilF) [Piscinibacter gummiphilus strain NS21]
MPVDTLVEAPQSSLSGVARVLVHAGKLNAKTAEDLVKSSKEKKTSFISSVIAGGAITSADLAHTLSAALALPVMDINAVD
LQRLPRNVIDTKLASQYQVLVLGKRGNRLFIGGADPTDQEAVERIKFATQLSPEWVILEHDKLAKILEDSGASATETLEN
IVGGDFEFDVSDEESAAPETQEVTVDVEDAPVVRFLQKMLIDAINSRASDLHFEPYEFHYRVRFRVDGELREITQPPIAI
KDKLASRIKVISRMDIAEKRVPQDGRMKLKFGNKSIDFRVSTLPTLFGEKIVIRILDSSSAKLGIEALGYEKVEKDRLLK
CIARPYGMVLVTGPTGSGKTVSLYTCLNILNQPGVNISTVEDPAEINLPGINQVNVNDKAGLTFSAALKSFLRQDPDVIM
VGEIRDLETADIAIKAAQTGHMVMSTLHTNDAPTTLTRLLNMGVAPFNIASSVLLITAQRLARRLCESCKAPAEYPKEAM
LKAGYKESDLDGSWKPYRAVGCSACTNGYKGRVGIYQVMPISEDIQRIILAEGTSMDIAEQAQREGIRDLRQSGLVKVRA
GFTTLEEVIAATNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=176847 A4W93_RS24650 WP_085753130.1 5390596..5392320(+) (pilF) [Piscinibacter gummiphilus strain NS21]
GTGCCCGTGGACACCCTTGTCGAAGCCCCCCAGTCGTCGTTGTCCGGCGTCGCACGCGTCCTGGTCCACGCTGGCAAGCT
GAACGCCAAGACGGCCGAGGACCTGGTCAAGTCCTCCAAGGAAAAGAAAACCAGCTTCATCTCGTCGGTGATCGCTGGCG
GCGCCATCACGTCCGCCGACCTGGCCCACACCCTGTCCGCCGCACTGGCCCTGCCGGTCATGGACATCAACGCGGTCGAC
CTGCAGCGGCTGCCGCGCAACGTCATCGACACCAAGCTCGCCAGCCAGTACCAGGTGCTGGTGCTCGGCAAGCGAGGCAA
CCGGCTCTTCATCGGCGGGGCGGATCCCACCGACCAGGAAGCGGTCGAACGCATCAAGTTCGCCACCCAGCTGTCGCCCG
AGTGGGTCATCCTCGAACACGACAAGCTCGCCAAGATCCTCGAAGACTCGGGCGCCAGCGCCACCGAAACCCTCGAGAAC
ATCGTCGGCGGCGACTTCGAGTTCGACGTCAGCGACGAGGAATCCGCCGCCCCCGAGACCCAGGAGGTCACGGTCGACGT
GGAAGACGCGCCGGTGGTGCGCTTCCTCCAGAAGATGCTGATCGACGCCATCAACTCCCGGGCGTCCGACCTCCACTTCG
AACCCTACGAGTTCCACTACCGCGTCCGCTTCCGCGTCGACGGCGAACTGCGCGAGATCACGCAGCCGCCCATCGCCATC
AAGGACAAGCTCGCCTCGCGCATCAAGGTCATCTCGCGCATGGACATCGCCGAAAAGCGGGTCCCGCAGGACGGCCGCAT
GAAGCTGAAGTTCGGCAACAAGTCCATCGACTTCCGGGTCAGCACGCTGCCCACGCTGTTCGGCGAGAAGATCGTGATCC
GGATTCTCGATTCCTCGAGCGCCAAGCTCGGCATCGAGGCCCTGGGCTACGAGAAGGTCGAGAAGGACCGCCTGCTCAAG
TGCATCGCACGCCCCTACGGCATGGTGCTCGTGACGGGCCCCACCGGCAGCGGCAAGACCGTGTCGCTCTACACCTGCCT
GAACATCCTGAACCAGCCGGGGGTGAACATCTCCACGGTGGAAGACCCGGCTGAAATCAACCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCCGGCCTCACCTTCTCGGCCGCCCTGAAATCCTTCCTCCGCCAGGATCCGGACGTCATCATG
GTCGGCGAAATCCGGGACCTGGAAACGGCCGACATCGCCATCAAGGCCGCCCAGACCGGCCACATGGTGATGTCCACGTT
GCACACGAACGACGCGCCCACCACCCTCACCCGGCTGCTGAACATGGGCGTGGCCCCGTTCAACATCGCCTCGAGCGTGC
TGCTGATCACCGCCCAGCGCCTGGCCCGCCGCCTCTGCGAATCGTGCAAGGCCCCGGCGGAGTACCCGAAGGAGGCCATG
CTGAAAGCGGGCTACAAGGAGTCCGACCTCGACGGATCCTGGAAACCCTACCGGGCCGTCGGCTGCTCGGCGTGCACGAA
TGGCTACAAAGGCCGGGTCGGCATTTACCAAGTGATGCCCATCAGCGAGGACATTCAGCGCATCATCCTGGCCGAGGGGA
CCTCGATGGACATCGCCGAGCAGGCCCAGCGCGAGGGGATCCGCGACCTGCGCCAGTCCGGCCTCGTCAAGGTGCGCGCC
GGTTTCACCACCCTCGAGGAGGTGATCGCCGCCACCAACGAGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-149)

Type II/IV secretion system protein (T2SSE)

(198-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W6LEY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.697

98.955

0.531
  pilB Acinetobacter baumannii D1279779

53.108

98.084

0.521
  pilB Acinetobacter baylyi ADP1

51.687

98.084

0.507
  pilB Legionella pneumophila strain ERS1305867

49.556

98.084

0.486
  pilB Vibrio cholerae strain A1552

45.246

98.955

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

45.081

97.387

0.439
  pilB Vibrio campbellii strain DS40M4

44.464

97.561

0.434
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.212

92.857

0.364

Multiple sequence alignment