Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   A4G13_RS07145 Genome accession   NZ_CP015031
Coordinates   1561861..1563246 (-) Length   461 a.a.
NCBI ID   WP_090655611.1    Uniprot ID   -
Organism   Basfia succiniciproducens strain JF4016     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1556861..1568246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4G13_RS07115 (A4G13_07090) - 1557097..1557819 (+) 723 WP_041640030.1 3-deoxy-D-manno-octulosonic acid kinase -
  A4G13_RS07120 (A4G13_07095) aqpZ 1557855..1558547 (-) 693 WP_090655618.1 aquaporin Z -
  A4G13_RS07125 (A4G13_07100) metJ 1558776..1559093 (+) 318 WP_041640032.1 met regulon transcriptional regulator MetJ -
  A4G13_RS07130 (A4G13_07105) - 1559164..1560009 (-) 846 WP_011201125.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  A4G13_RS07135 (A4G13_07110) aroB 1560022..1561110 (-) 1089 WP_011201126.1 3-dehydroquinate synthase -
  A4G13_RS07140 (A4G13_07115) aroK 1561140..1561667 (-) 528 WP_090655615.1 shikimate kinase AroK -
  A4G13_RS07145 (A4G13_07120) comE 1561861..1563246 (-) 1386 WP_090655611.1 type IV pilus secretin PilQ Machinery gene
  A4G13_RS07150 (A4G13_07125) - 1563261..1563671 (-) 411 WP_090655608.1 pilus assembly protein PilP -
  A4G13_RS07155 (A4G13_07130) - 1563674..1564186 (-) 513 WP_090655605.1 hypothetical protein -
  A4G13_RS07160 (A4G13_07135) - 1564164..1564700 (-) 537 WP_011201131.1 hypothetical protein -
  A4G13_RS07165 (A4G13_07140) - 1564702..1565529 (-) 828 WP_090655602.1 hypothetical protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50752.08 Da        Isoelectric Point: 6.7337

>NTDB_id=176326 A4G13_RS07145 WP_090655611.1 1561861..1563246(-) (comE) [Basfia succiniciproducens strain JF4016]
MESIISFGKKCGLFFGIFISSAFAGESGTFAERQFSIHLKKAPLVATLQQLALEQNANLVIDDELEGTLSLKLEKVNLER
LFHSVAKLKNLSLHKDKDIYYFTKNNLIEPSSIAGELKNTENFTALSEPNLVSTTVKLHFAKASEVMKSLTSGTGSLLSP
VGSVSFDERSNQLLIQDDRRSLQNIKNIIAQLDKPIEQIAIEARIVTMNDESLKELGVRWGLLEGVNSAHRIAGSLEANG
FADIGQNLNVNFPTSATPAGSVALQVAKIHGRLLDLELTALEQENNVKIIASPRLLTTNKKSASIKQGTEIPYVAVNRKN
DTEHVEFREAVLGLEVTPHISKDNSILLDLIVSQNSPGANIVYGNGNLISIDKQEINTQVFAKDGETIVLGGVFNDTITK
SEDKVPILGDIPLIKHLFSKESEKHQKRELVIFVTPHILKQGESLEQIQKRFKYAPKSPEK

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=176326 A4G13_RS07145 WP_090655611.1 1561861..1563246(-) (comE) [Basfia succiniciproducens strain JF4016]
ATGGAAAGCATAATAAGTTTTGGTAAAAAGTGCGGTCTGTTTTTTGGAATTTTTATTTCTTCCGCATTTGCCGGGGAATC
CGGTACGTTTGCGGAACGGCAATTTTCCATTCACTTGAAAAAGGCGCCGTTGGTGGCGACCTTACAACAGCTTGCTTTAG
AACAAAATGCTAATCTGGTGATTGATGACGAACTGGAAGGTACATTGTCGTTAAAGCTTGAAAAGGTGAATCTGGAGCGT
CTTTTTCATTCGGTGGCAAAGCTGAAAAATCTTAGCTTACATAAAGACAAGGATATTTATTATTTTACGAAAAATAACCT
GATTGAACCGTCTTCCATCGCCGGTGAGCTAAAAAATACGGAAAATTTTACCGCACTTTCGGAACCTAATCTGGTTAGTA
CTACCGTCAAGCTGCATTTTGCCAAAGCCTCGGAGGTGATGAAATCATTAACATCGGGAACGGGTTCATTGCTTTCGCCC
GTTGGTTCCGTCAGCTTTGACGAACGCAGTAACCAGCTGCTTATTCAAGATGATCGCAGATCGTTGCAAAATATCAAAAA
TATAATCGCTCAATTAGATAAACCTATCGAACAAATCGCAATTGAAGCCCGTATCGTGACGATGAACGACGAAAGTTTAA
AAGAACTCGGTGTGCGTTGGGGGCTGTTGGAGGGGGTAAATAGCGCACATCGTATTGCCGGCAGCCTGGAAGCTAACGGA
TTCGCCGATATAGGGCAAAACCTTAACGTTAATTTCCCGACCAGTGCGACGCCGGCAGGTTCCGTAGCGTTGCAGGTGGC
AAAAATTCACGGGCGTTTGCTGGATTTGGAATTAACCGCTTTAGAGCAGGAAAACAATGTCAAGATCATTGCCAGCCCGC
GGCTGCTGACTACCAATAAGAAAAGTGCGAGTATTAAACAAGGAACGGAAATTCCTTATGTGGCGGTGAATCGTAAAAAT
GATACGGAACACGTGGAGTTTCGAGAGGCGGTGCTAGGGTTGGAAGTCACTCCGCATATTTCAAAAGATAATTCTATTTT
ACTTGACCTTATCGTAAGCCAGAATTCTCCGGGCGCAAATATTGTTTATGGGAATGGCAATTTAATTTCCATTGATAAAC
AGGAAATTAATACGCAGGTTTTTGCAAAAGACGGCGAAACCATCGTATTAGGCGGCGTATTTAACGATACTATAACGAAA
AGCGAAGATAAAGTGCCGATTTTGGGCGATATTCCGCTGATAAAACATTTATTCAGCAAAGAAAGCGAAAAACACCAAAA
ACGGGAATTGGTTATTTTTGTTACGCCTCATATATTAAAACAAGGCGAAAGCTTGGAGCAAATACAAAAACGTTTTAAAT
ATGCGCCAAAATCTCCTGAAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(135-198)

Bacterial type II and III secretion system protein (Secretin)

(280-440)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

66.516

95.879

0.638
  comE Haemophilus influenzae 86-028NP

65.611

95.879

0.629
  comE Glaesserella parasuis strain SC1401

51.869

92.842

0.482
  pilQ Vibrio campbellii strain DS40M4

40.984

92.625

0.38
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.281

92.625

0.373
  pilQ Vibrio cholerae strain A1552

40.281

92.625

0.373

Multiple sequence alignment