Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   A4G16_RS06675 Genome accession   NZ_CP015030
Coordinates   1401282..1402586 (+) Length   434 a.a.
NCBI ID   WP_165889234.1    Uniprot ID   A0A6G8JJ07
Organism   Mannheimia granulomatis strain B 234/94     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1396282..1407586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4G16_RS06650 (A4G16_06545) - 1396440..1398995 (-) 2556 WP_165889229.1 penicillin-binding protein 1A -
  A4G16_RS06655 (A4G16_06550) - 1399120..1399830 (+) 711 WP_165889230.1 competence protein ComA -
  A4G16_RS06660 (A4G16_06555) - 1399806..1400324 (+) 519 WP_165889231.1 hypothetical protein -
  A4G16_RS06665 (A4G16_06560) - 1400328..1400873 (+) 546 WP_165889232.1 hypothetical protein -
  A4G16_RS06670 (A4G16_06565) - 1400866..1401273 (+) 408 WP_165889233.1 hypothetical protein -
  A4G16_RS06675 (A4G16_06570) comE 1401282..1402586 (+) 1305 WP_165889234.1 type IV pilus secretin PilQ Machinery gene
  A4G16_RS06680 (A4G16_06575) nusB 1402663..1403076 (+) 414 WP_027074137.1 transcription antitermination factor NusB -
  A4G16_RS06685 (A4G16_06580) thiL 1403140..1404120 (+) 981 WP_165889235.1 thiamine-phosphate kinase -
  A4G16_RS06690 (A4G16_06585) - 1404126..1404599 (+) 474 WP_165889236.1 phosphatidylglycerophosphatase A -
  A4G16_RS06695 (A4G16_06590) - 1404607..1405233 (+) 627 WP_165889237.1 LysE family transporter -
  A4G16_RS06700 (A4G16_06595) ruvA 1405344..1405949 (+) 606 WP_165889238.1 Holliday junction branch migration protein RuvA -
  A4G16_RS06705 (A4G16_06600) ruvB 1405960..1406964 (+) 1005 WP_165889239.1 Holliday junction branch migration DNA helicase RuvB -

Sequence

Protein


Download         Length: 434 a.a.        Molecular weight: 48031.22 Da        Isoelectric Point: 7.6315

>NTDB_id=176303 A4G16_RS06675 WP_165889234.1 1401282..1402586(+) (comE) [Mannheimia granulomatis strain B 234/94]
MSKILSLLLLTPSLVLANTLSISLKNAPTPAILTYLAEETDKNIVLEDNIDTKTTLRLDNKSIDEILKTISKINKLSLKQ
ENDIAYINKQENKLESMPPIAHLQNNGQIHPLAPPKLITKTIKLYYAKASEIIDSLTKGSGTFLSENGYIHFDERSNSLI
IKDSEKSLRNIEKLVKKLDHPTEQIAIEARIVTISSEHLQELGVRWGMFSSSPEHYKFGGKLEGNGLNNMANNLNVNFPV
ANGASAVLQIASINSRVLDLELSALEQENSVEIIASPRLLTTNKKAASIKQGTEIPYVMYNTKSEATDVEFKEAVLGLEV
TPHLSTQNQILLDLVVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFIKRLF
SQTRDKISKRELVIFVTPYILQSSEKISTISKLK

Nucleotide


Download         Length: 1305 bp        

>NTDB_id=176303 A4G16_RS06675 WP_165889234.1 1401282..1402586(+) (comE) [Mannheimia granulomatis strain B 234/94]
ATGTCTAAAATTCTCTCATTATTATTGCTTACCCCTTCTCTTGTCTTAGCAAATACATTATCTATTTCATTAAAAAATGC
CCCAACACCTGCAATATTGACTTATTTAGCAGAAGAAACCGATAAAAATATTGTATTGGAAGATAATATAGACACTAAAA
CCACCTTACGTTTAGACAATAAATCTATTGATGAAATATTAAAAACAATAAGTAAAATCAATAAGTTATCATTAAAGCAA
GAAAACGACATTGCTTATATTAATAAACAAGAAAATAAGTTAGAAAGTATGCCACCAATCGCTCATTTGCAAAATAATGG
GCAAATTCACCCGCTTGCTCCCCCAAAACTTATCACGAAAACGATCAAACTCTATTATGCTAAAGCTTCTGAAATCATAG
ACTCTTTAACTAAAGGCAGTGGTACATTTCTATCGGAAAACGGCTATATTCATTTTGACGAAAGAAGTAATAGTTTGATT
ATTAAAGACAGCGAAAAGTCGTTACGAAATATAGAAAAATTAGTAAAAAAACTCGATCATCCAACTGAGCAAATTGCTAT
AGAGGCCAGAATTGTAACGATTAGCAGCGAGCATCTACAAGAGCTTGGTGTACGCTGGGGTATGTTTTCCTCAAGTCCGG
AGCATTATAAATTTGGCGGAAAGCTGGAAGGCAATGGTTTGAACAATATGGCTAACAATTTAAATGTCAATTTCCCAGTA
GCAAATGGTGCTTCTGCAGTATTACAAATTGCCTCCATTAATAGCCGTGTACTAGATTTGGAGCTTTCTGCACTTGAGCA
AGAAAACAGTGTAGAAATTATTGCAAGCCCACGCCTACTCACTACCAATAAAAAAGCCGCCAGCATTAAGCAAGGTACGG
AAATCCCTTATGTAATGTATAACACAAAATCAGAAGCAACTGATGTTGAATTTAAAGAAGCCGTATTAGGACTGGAAGTT
ACCCCTCACCTTTCCACACAAAATCAGATTTTACTGGATCTGGTTGTAACTCAAAATTCTCCTAATTCGCAATCCGGTAG
CAGTGGTTTAATTACCATAGATAAACAAGAATTGAATACACAAGTCTTTGCCAAACACGGAGAAACCATTGTTTTAGGTG
GTATTTTCCAACATTTAACCCAAAAAGGAGAAGATAAAGTACCAATTTTAGGCTCAATTCCCTTTATTAAGCGGCTGTTT
AGTCAAACACGAGATAAAATCAGTAAACGTGAATTAGTCATTTTCGTTACCCCTTATATTTTACAATCTAGTGAGAAAAT
CAGTACCATCTCAAAATTAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-421)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-184)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8JJ07

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

65.34

98.387

0.643
  comE Haemophilus influenzae Rd KW20

50.917

100

0.512
  comE Haemophilus influenzae 86-028NP

50.229

100

0.505
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.192

97.005

0.38
  pilQ Vibrio cholerae strain A1552

39.192

97.005

0.38
  pilQ Vibrio campbellii strain DS40M4

38.263

98.157

0.376

Multiple sequence alignment