Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   A2I97_RS11830 Genome accession   NZ_CP014990
Coordinates   2445595..2446665 (-) Length   356 a.a.
NCBI ID   WP_070082113.1    Uniprot ID   -
Organism   Bacillus velezensis strain KD1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2440595..2451665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A2I97_RS11780 (A2I97_11415) sipW 2440655..2441239 (-) 585 WP_012117977.1 signal peptidase I SipW -
  A2I97_RS11785 (A2I97_11420) tapA 2441211..2441882 (-) 672 WP_070082109.1 amyloid fiber anchoring/assembly protein TapA -
  A2I97_RS11790 (A2I97_11425) - 2442141..2442470 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  A2I97_RS11795 (A2I97_11430) - 2442510..2442689 (-) 180 WP_003153093.1 YqzE family protein -
  A2I97_RS11800 (A2I97_11435) comGG 2442746..2443123 (-) 378 WP_070082110.1 competence type IV pilus minor pilin ComGG Machinery gene
  A2I97_RS11805 (A2I97_11440) comGF 2443124..2443519 (-) 396 WP_070082111.1 competence type IV pilus minor pilin ComGF -
  A2I97_RS11810 (A2I97_11445) comGE 2443533..2443847 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  A2I97_RS11815 (A2I97_11450) comGD 2443831..2444268 (-) 438 WP_070082112.1 competence type IV pilus minor pilin ComGD Machinery gene
  A2I97_RS11820 (A2I97_11455) comGC 2444258..2444566 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  A2I97_RS11825 (A2I97_11460) comGB 2444571..2445608 (-) 1038 WP_063174751.1 competence type IV pilus assembly protein ComGB Machinery gene
  A2I97_RS11830 (A2I97_11465) comGA 2445595..2446665 (-) 1071 WP_070082113.1 competence type IV pilus ATPase ComGA Machinery gene
  A2I97_RS11835 (A2I97_11470) - 2446857..2447807 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  A2I97_RS11840 (A2I97_11475) - 2447953..2449254 (+) 1302 WP_070082114.1 hemolysin family protein -
  A2I97_RS11845 (A2I97_11480) - 2449292..2450122 (-) 831 WP_070082115.1 STAS domain-containing protein -
  A2I97_RS11855 (A2I97_11490) - 2450328..2450708 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  A2I97_RS11860 (A2I97_11495) - 2450945..2451190 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.91 Da        Isoelectric Point: 9.5862

>NTDB_id=175783 A2I97_RS11830 WP_070082113.1 2445595..2446665(-) (comGA) [Bacillus velezensis strain KD1]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFREAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=175783 A2I97_RS11830 WP_070082113.1 2445595..2446665(-) (comGA) [Bacillus velezensis strain KD1]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC
ACAAGGGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATCGCTGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCGGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

81.742

100

0.817
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment