Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   A3224_RS01050 Genome accession   NZ_CP014864
Coordinates   244660..245736 (+) Length   358 a.a.
NCBI ID   WP_067150327.1    Uniprot ID   A0A143HI08
Organism   Microbulbifer thermotolerans strain DAU221     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 239660..250736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3224_RS01040 (A3224_01040) - 241222..241704 (+) 483 WP_067150321.1 chemotaxis protein CheW -
  A3224_RS01045 (A3224_01045) - 241825..244470 (-) 2646 WP_067150324.1 penicillin-binding protein 1A -
  A3224_RS01050 (A3224_01050) pilM 244660..245736 (+) 1077 WP_067150327.1 pilus assembly protein PilM Machinery gene
  A3224_RS01055 (A3224_01055) - 245738..246313 (+) 576 WP_067150330.1 PilN domain-containing protein -
  A3224_RS01060 (A3224_01060) - 246326..246946 (+) 621 WP_067150333.1 type 4a pilus biogenesis protein PilO -
  A3224_RS01065 (A3224_01065) - 246943..247470 (+) 528 WP_082817342.1 pilus assembly protein PilP -
  A3224_RS01070 (A3224_01070) pilQ 247473..249677 (+) 2205 WP_067150340.1 type IV pilus secretin PilQ -
  A3224_RS01075 (A3224_01075) - 249913..250428 (+) 516 WP_074902189.1 shikimate kinase -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 38811.17 Da        Isoelectric Point: 4.3247

>NTDB_id=174809 A3224_RS01050 WP_067150327.1 244660..245736(+) (pilM) [Microbulbifer thermotolerans strain DAU221]
MGMLPFLNKGPKAMLGLDISSTAVKLLELSRNGDRYRVECYAVEPLPPNAVVDKNINDVGATAEAIRKVVRRSKTRLRQA
AVAVSGSAVITKTLEMPADLSDDDLEARIALEAEQYIPFSLDEVNLDFEVQGPSAKGEGQVEVLLAACRSETVEQRVSTL
TEAELQAGVVDVEAYAIERAYTLMERQFPPQEQLVVAVIDIGATMTALSVLVDGKTVYTREQLFGGRQLTDEIQRRYGLS
SEEAGLAKRQGGSGLPDDYYPEVLEPFLDAVVQQVTRSLQFFYSSSSYSDVDYILLGGGVAAMDGLADMVGQKLNKPTLV
ANPFLDMAVASRVNSQALASEAPSLMIAAGLAMREKEY

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=174809 A3224_RS01050 WP_067150327.1 244660..245736(+) (pilM) [Microbulbifer thermotolerans strain DAU221]
ATGGGGATGCTCCCTTTCCTCAACAAAGGCCCCAAGGCCATGCTGGGACTCGATATCAGCTCCACCGCTGTCAAGCTGCT
GGAGCTCAGTCGCAACGGCGACCGGTATCGGGTCGAGTGTTATGCCGTGGAGCCTCTGCCGCCCAATGCGGTGGTGGACA
AAAATATCAACGACGTGGGCGCGACCGCGGAGGCTATCCGCAAGGTAGTGCGCCGTTCCAAGACCCGTCTGCGCCAGGCG
GCAGTGGCTGTATCCGGCTCCGCGGTGATTACCAAAACTCTGGAAATGCCAGCGGATCTCTCCGATGATGATCTGGAGGC
CCGGATAGCGCTGGAAGCAGAACAATACATTCCTTTTTCGCTCGACGAAGTAAACCTGGATTTTGAGGTGCAGGGGCCAT
CGGCCAAAGGCGAGGGCCAAGTGGAAGTTCTGCTGGCCGCTTGTCGCAGCGAGACGGTCGAGCAGCGGGTTTCCACTCTG
ACCGAAGCTGAACTCCAGGCGGGGGTGGTGGATGTGGAAGCCTACGCGATTGAACGGGCTTACACACTGATGGAGCGCCA
GTTCCCGCCCCAGGAGCAACTGGTGGTTGCGGTGATTGATATTGGCGCGACGATGACCGCGCTTTCGGTGCTGGTGGACG
GCAAGACCGTCTACACTCGCGAACAGTTGTTTGGCGGCCGCCAGCTAACCGATGAAATCCAGCGCCGTTACGGTCTGTCT
TCGGAAGAGGCCGGTTTAGCCAAGCGCCAGGGCGGTAGCGGCTTGCCGGACGATTACTATCCGGAGGTGCTGGAGCCTTT
CCTCGATGCCGTGGTGCAACAAGTCACGCGCTCTCTACAATTTTTCTACTCGTCCTCTTCCTACAGCGACGTCGACTACA
TACTGCTTGGCGGCGGTGTAGCGGCGATGGATGGACTGGCCGATATGGTCGGTCAGAAACTCAACAAGCCGACATTGGTG
GCCAATCCTTTCCTCGATATGGCGGTGGCCTCTCGGGTCAACAGCCAGGCGCTGGCCAGCGAAGCTCCGTCGCTGATGAT
TGCCGCCGGCCTGGCGATGCGGGAGAAGGAGTACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A143HI08

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.989

98.324

0.511
  comM Acinetobacter nosocomialis M2

51.989

98.324

0.511
  comM Acinetobacter baylyi ADP1

48.011

98.324

0.472
  pilM Legionella pneumophila strain ERS1305867

43.258

99.441

0.43

Multiple sequence alignment